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In relation to this article, we declare that there is no conflict of interest.
Publication history
Received May 27, 2011
Accepted June 15, 2011
articles This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Mathematical analysis of colonial formation of embryonic stem cells in microfluidic system

Department of Biological Engineering, Inha University, Incheon 402-751, Korea 1Departmet of Chemical Engineering & Nano-Bio Technology, Hannam University, Daejeon 305-811, Korea
hsshin@inha.ac.kr
Korean Journal of Chemical Engineering, March 2012, 29(3), 392-395(4), 10.1007/s11814-011-0181-7
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Abstract

A fluidic environment affects mechanochemical characteristics of embryonic stem cells (ESCs). Perfusion is recognized as an attractive culture mode of ESCs since the steady fluidic state can enhance ESCs’ controllability, supporting a unique cell culture condition. Cellular membrane motility presents important information about cellular dynamics such as adhesion, spreading, and migration. Thus, an investigation of the perfusion-induced membrane motility is significant to understand the mechanochemical behavior of ESCs in the steady culture state. In this research,_x000D_ we suggest Lfr, the ratio of circumferential membrane unattached to other cells’ to the cell’s circumference, as a new parameter to characterize cells’ shape and motility. Lfr of embryonic stem cells has positive correlations with cellular area (Ar) and free peripheral length (Lf) but a negative correlation with roundness (Rn). We also propose a mathematical model representing ESCs’ membrane motilities and demonstrate their colonical behavior.

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