Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received May 30, 2011
Accepted October 24, 2011
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Enhancement of transglutaminase production in Streptomyces mobaraensis DSM 40587 by non-nutritional stress conditions: Effects of heat shock, alcohols, and salt treatments
Lili Zhang
Lanwei Zhang†
Huaxi Yi
Ming Du
Yingchun Zhang
Xue Han
Zhen Feng1
Jingyan Li
Yuehua Jiao
Yanhe Zhang2
Chunfeng Guo
School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China 1College of Food Science, Northeast Agricultural University, Harbin 150090, China 2National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Korean Journal of Chemical Engineering, July 2012, 29(7), 913-917(5), 10.1007/s11814-011-0274-3
Download PDF
Abstract
Stress-mediated bioprocess is a strategy designed to enhance biological target productivity. In this study, an attempt was made to enhance transglutaminase (TGase) production by Streptomyces mobaraensis by using different stress conditions including heat shock, alcohols and salt stress. Results showed that the effects of stress on TGase production depended on the type applied. For heat shock, TGase production (1.32 U/ml) was recorded maximum in the culture treated at 48 h post inoculation in water bath at 60 ℃ for 1 min. For alcohols treatment, the maximum activity of TGase (1.77 and 1.75 U/ml) was obtained when 3% methanol was added into the medium at 0 or 24 h of fermentation. However, a 3.5-fold increased production of TGase (3.8 U/ml) was observed in the medium supplemented with 0.2mol/l MgCl2 compared with the basic medium at the beginning of fermentation. In conclusion, TGase production from S. mobaraensis was improved by heat shock, methanol and salt stress treatments, MgCl2 stress was the most effective.
Keywords
References
Ando H, Adachi M, Umeda K, Matsuura A, Nonaka M, Uchio R,Tanaka H, Motoki M, Agric. Biol. Chem., 53, 2613 (1989)
Beganovic J, Pavunc AL, Gjuracic K, Spoljarec M, Suskovic J, Kos B, J. Food Sci., 76, M124 (2011)
Farnsworth JP, Li J, Hendricks GM, Guo MR, Small Ruminant Res., 65, 113 (2006)
Jiang SJ, Zhao XH, Eur. Food Res. Technol., 231, 679 (2010)
Wang HW, Kim IH, Park CS, Lee JH, Korean J. Chem. Eng., 25(4), 801 (2008)
Suzuki S, Izawa Y, Kobayashi K, Eto Y, Yamanaka S, Kubota K, Yokozeki K, Biosci. Biotechnol. Biochem., 64, 2344 (2000)
Iranzo M, Aguado C, Pallotti C, Canizares JA, Mormeneo S, Microbiol-Sgm., 148, 1329 (2002)
Yan GL, Du GC, Li Y, Chen J, Zhong JJ, Process Biochem., 40, 963 (2005)
Zhu Y, Rinzema A, Tramper J, Bol J, Biotechnol. Bioeng., 50(3), 291 (1996)
Zheng MY, Du GC, Chen J, Lun SY, World J. Microb. Biot., 18, 767 (2002)
de Souza CFV, Flores SH, Ayub MAZ, Process Biochem., 41, 1186 (2006)
Tellez-Luis SJ, Ramirez JA, Vazquez M, Food Technol. Biotechnol., 42, 75 (2004)
Umakoshi H, Yoshimoto M, Shimanouchi T, Kuboi R, Komasawa I, Biotechnol. Prog., 14(2), 218 (1998)
Kang DH, Jeh EJ, Seo JW, Chun BH, Hur BK, Korean J. Chem. Eng., 24(4), 651 (2007)
Rigali S, Titgemeyer F, Barends S, Mulder S, Thomae AW, Hopwood DA, van Wezel GP, EMBO Rep., 9, 670 (2008)
Doull JL, Singh AK, Hoare M, Ayer SW, J. Ind. Microbiol., 13, 120 (1994)
Nakata K, Yoshimoto A, Yamada Y, Biosci. Biotechnol. Biochem., 63, 293 (1999)
Himabindu M, Potumarthi R, Jetty A, Process Biochem., 42, 1352 (2007)
Folk JE, Cole PW, J. Biol. Chem., 241, 5518 (1966)
Bradford MM, Anal. Biochem., 72, 248 (1976)
Ngo KX, Umakoshi H, Shimanouchi T, Jung HS, Morita S, Kuboi R, J. Biosci. Bioeng., 100(5), 495 (2005)
Fernandez MJ, Adrio JL, Piret JM, Wolfe S, Ro S, Demain AL, Appl. Microbiol. Biotechnol., 52(4), 484 (1999)
Haq IU, Ali S, Qadeer MA, Lqbal J, Bioresour. Technol., 86(3), 227 (2003)
Novotna J, Vohradsky J, Berndt P, Gramajo H, Langen H, Li XM, Minas W, Orsaria L, Roeder D, Thompson CJ, Mol. Microbiol., 48, 1289 (2003)
Viollier PH, Kelemen GH, Dale GE, Nguyen KT, Buttner MJ, Thompson CJ, Mol. Microbiol., 47, 699 (2003)
Vohradsky J, Li XM, Dale G, Folcher M, Nguyen L, Viollier PH, Thompson CJ, J. Bacteriol., 182, 4979 (2000)
Wang C, Long X, Mao X, Dong H, Xu L, Li Y, Microbiol.Res., 165, 221 (2010)
Beganovic J, Pavunc AL, Gjuracic K, Spoljarec M, Suskovic J, Kos B, J. Food Sci., 76, M124 (2011)
Farnsworth JP, Li J, Hendricks GM, Guo MR, Small Ruminant Res., 65, 113 (2006)
Jiang SJ, Zhao XH, Eur. Food Res. Technol., 231, 679 (2010)
Wang HW, Kim IH, Park CS, Lee JH, Korean J. Chem. Eng., 25(4), 801 (2008)
Suzuki S, Izawa Y, Kobayashi K, Eto Y, Yamanaka S, Kubota K, Yokozeki K, Biosci. Biotechnol. Biochem., 64, 2344 (2000)
Iranzo M, Aguado C, Pallotti C, Canizares JA, Mormeneo S, Microbiol-Sgm., 148, 1329 (2002)
Yan GL, Du GC, Li Y, Chen J, Zhong JJ, Process Biochem., 40, 963 (2005)
Zhu Y, Rinzema A, Tramper J, Bol J, Biotechnol. Bioeng., 50(3), 291 (1996)
Zheng MY, Du GC, Chen J, Lun SY, World J. Microb. Biot., 18, 767 (2002)
de Souza CFV, Flores SH, Ayub MAZ, Process Biochem., 41, 1186 (2006)
Tellez-Luis SJ, Ramirez JA, Vazquez M, Food Technol. Biotechnol., 42, 75 (2004)
Umakoshi H, Yoshimoto M, Shimanouchi T, Kuboi R, Komasawa I, Biotechnol. Prog., 14(2), 218 (1998)
Kang DH, Jeh EJ, Seo JW, Chun BH, Hur BK, Korean J. Chem. Eng., 24(4), 651 (2007)
Rigali S, Titgemeyer F, Barends S, Mulder S, Thomae AW, Hopwood DA, van Wezel GP, EMBO Rep., 9, 670 (2008)
Doull JL, Singh AK, Hoare M, Ayer SW, J. Ind. Microbiol., 13, 120 (1994)
Nakata K, Yoshimoto A, Yamada Y, Biosci. Biotechnol. Biochem., 63, 293 (1999)
Himabindu M, Potumarthi R, Jetty A, Process Biochem., 42, 1352 (2007)
Folk JE, Cole PW, J. Biol. Chem., 241, 5518 (1966)
Bradford MM, Anal. Biochem., 72, 248 (1976)
Ngo KX, Umakoshi H, Shimanouchi T, Jung HS, Morita S, Kuboi R, J. Biosci. Bioeng., 100(5), 495 (2005)
Fernandez MJ, Adrio JL, Piret JM, Wolfe S, Ro S, Demain AL, Appl. Microbiol. Biotechnol., 52(4), 484 (1999)
Haq IU, Ali S, Qadeer MA, Lqbal J, Bioresour. Technol., 86(3), 227 (2003)
Novotna J, Vohradsky J, Berndt P, Gramajo H, Langen H, Li XM, Minas W, Orsaria L, Roeder D, Thompson CJ, Mol. Microbiol., 48, 1289 (2003)
Viollier PH, Kelemen GH, Dale GE, Nguyen KT, Buttner MJ, Thompson CJ, Mol. Microbiol., 47, 699 (2003)
Vohradsky J, Li XM, Dale G, Folcher M, Nguyen L, Viollier PH, Thompson CJ, J. Bacteriol., 182, 4979 (2000)
Wang C, Long X, Mao X, Dong H, Xu L, Li Y, Microbiol.Res., 165, 221 (2010)
