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Received June 23, 2011
Accepted October 24, 2011
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Production of cellulases and β-glucosidase in Trichoderma reesei mutated by proton beam irradiation
Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea 1Center for Environmental Technology Research, Korea Institute of Science and Technology, Seoul 136-130, Korea
kimsw@korea.ac.kr
Korean Journal of Chemical Engineering, July 2012, 29(7), 925-930(6), 10.1007/s11814-011-0272-5
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Abstract
To obtain mutant strains producing high levels of cellulases (FPase and CMCase) and β-glucosidase, Trichoderma reesei KCTC 6950 was mutated by proton beam irradiation. Five mutants were selected out of 1,000 mutants of T.reesei treated with proton beam irradiation, based on their ability for enzyme production on a plate screening medium. In submerged cultures containing Mandel’s fermentation medium, the mutant strain T-2 (MT-2) demonstrated a 165% increase in the activity of FPase, a 146% increase in the activity of CMCase, and a 313% increase in the activity of β-glucosidase, compared with the wild type strain. Additionally, the properties of high level β-glucosidase produced by MT-2 were the same as those of the wild type strain, e.g., an optimum pH of 4.8, and an optimum temperature of 65 ℃. Moreover, the protein concentrations of β-glucosidase produced by the wild type strain and MT-2 were measured by SDS-PAGE, and then β-glucosidase activities were detected by the MUG-zymogram assay.
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