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Received May 30, 2011
Accepted November 2, 2011
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Ethanol production by co-fermentation of hexose and pentose from food wastes using Saccharomyces coreanus and Pichia stipitis
Department of Environmental Engineering, The University of Seoul, 13, Siripdae-gil, Dongdaemun-gu, Seoul 130-743, Korea 1Department of Marine Environmental Engineering, Mokpo National Maritime University, 91, Haeyangdaehak-ro, Mokpo-gu, Jeonnam 530-729, Korea
Korean Journal of Chemical Engineering, August 2012, 29(8), 1038-1043(6), 10.1007/s11814-011-0282-3
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Abstract
To improve the conversion rate of a saccharification liquid from food wastes containing pentoses and hexoses into bioethanol, after selecting Saccharomyces coreanus and Pichia stipitis, the respective fermentation and co-fermentation properties were investigated. In the fermentation using S. coreanus, the result under anaerobic condition was better than under aerobic conditions. In the anaerobic fermentation, the concentration of the reducing sugar and glucose remaining_x000D_
after 24 hrs was 9.09 and 1.88 g/L, respectively, with 40.59 g/L of ethanol produced; the ethanol productivity was 1.69 g/L-h. Also, even with the fermentation using P. stipitis, the reducing sugars and glucose were rapidly reduced, with a marked production of ethanol, but the ethanol produced was lower than those under anaerobic and aerobic conditions with the use of S. coreanus. Therefore, for the production of a high concentration of bioethanol from food wastes, ethanol fermentation was induced using S. coreanus until the middle of the fermentation, with P. stipitis used during the latter stage of the fermentation, where the circumstance favored its use, and thus, the carbon source not converted by S. coreanus was later converted to ethanol. As a result, both ethanol production of 48.63 g/L and productivity of 2.03 g/L-h increased over those of the anaerobic fermentation using S. coreanus.
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References
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Najafpour G, Younesi H, Ismail KSK, Bioresour. Technol., 92(3), 251 (2004)
Grootjen DRJ, van Der Lans RGJM, Luyben KCAM, Enzyme Microb. Technol., 13, 648 (1991)
Jeong SM, Jin GS, Kim YJ, Kim SJ, Lee DH, J. Kor. Soc. Waste Manage., 26, 419 (2009)
Jeong SM, Jin GS, Kim YJ, Lee DH, J. Kor. Soc. Waste Manage., 27, 130 (2010)
Jin GS, Jeong SM, Kim YJ, Lee DH, J. Kor. Soc. Waste Manage., 27, 438 (2010)
Gulati M, Westgate PJ, Brewer M, Hendrickson R, Ladisch R, Appl. Biochem. Biotechnol., 57/58, 103 (1996)
Barnett JA, Payne RW, Yarrow D, A guide to identifying and classifying yeasts, Cambridge Univ. Press, London (1979)
Jeffries TW, Adv. Biochem. Eng. Biotechnol., 27, 1 (1983)
Dellweg H, Rizzi M, Methner H, Debus D, Biotechnol. Lett., 6, 395 (1984)
Slininger PJ, Bothast RJ, Okos MR, Ladish MR, Biotechnol. Lett., 7, 431 (1985)
du Preez JC, van der Walt JP, Biotechnol. Lett., 5, 357 (1983)
Schneider H, Wang PY, Chan YK, Maleszka R, Biotechnol. Lett., 3, 89 (1981)
Slininger PJ, Bothast RJ, Van Cauwenberge JE, Kurtzman CP, Biotechnol. Bioeng., 24, 371 (1982)
Laplace JM, Delgenes JP, Moletta R, Navarro JM, J. Ferment. Bioeng., 75, 207 (1993)
Grootjen DRJ, Jansen ML, van der Lans RGJM, Enzyme Microb. Technol., 13, 828 (1991)
Lebeau T, Jouenne T, Junter GA, Microbiol. Res., 162, 211 (2007)
Lodder J, The Yeast, North-Holland Publishing Company-Amsterdam, London a Taxonomic Study (1970)
Barnett JA, Payne RW, Yarrow D, Yeasts, characteristics and identification, Cambridge University Press (1983)
Yu TS, J. Inst. Nat. Sci., 22, 47 (2003)
Slininger PJ, Bothast RJ, Ladisch MR, Okos MR, Biotechnol. Bioeng., 35, 727 (1990)
Miller GL, Anal. Chem., 31, 426 (1959)
Laplace JM, Delgenes JP, Moletta R, Navarro JM, Appl.Microbiol. Biotechnol., 36, 158 (1991)
Seo JH, Liquor. Ind., 14, 33 (1994)
Kwon SI, Liquor. Ind., 23, 35 (2003)
Ministry of Knowledge Economy, Republic of Korea, Development of ethanol fermentation process using pentose (1995)
Hahn-Hagerdal B, Karhumaa K, Fonseca C, Spencer-Martins I, Gorwa-Grauslund MF, Appl. Microbiol. Biotechnol., 74(5), 937 (2007)
Kilian SG, van Uden N, Appl. Microbiol. Biotechnol., 27, 545 (1988)
du Preez JC, Bosch M, Prior BA, Appl. Microbiol. Biotechnol., 23, 228 (1986)
Vallet C, Said R, Rabiller C, Martin ML, Bioorganic Chemistry., 24, 319 (1996)
Jeong SM, Kwon JA, Jin GS, Kim YJ, Kim SJ, Lee DH, Jeon EJ, Lee H, Proc. Autumn Conf. J. Kor. Soc. Waste Manage., 141 (2009)
Najafpour G, Younesi H, Ismail KSK, Bioresour. Technol., 92(3), 251 (2004)
Grootjen DRJ, van Der Lans RGJM, Luyben KCAM, Enzyme Microb. Technol., 13, 648 (1991)