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Received August 8, 2011
Accepted November 2, 2011
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Self-assembly electrode based on silver nanoparticle toward electrogenerated chemiluminescence analysis of glucose
Chemical Engineering Department, Faculty of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran 1Chemical Engineering Department, Faculty of Engineering, Tehran South Branch, Islamic Azad University, Tehran, Iran 2Nanobiotechnology Research Group, Babol Noshirvani University of Technology, Babol, Iran
A.Shokuhi@gmail.com
Korean Journal of Chemical Engineering, August 2012, 29(8), 1063-1068(6), 10.1007/s11814-011-0280-5
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Abstract
Electrogenerated Chemiluminescence (ECL) involves applying a certain electric potential to a chemical reaction, resulting in the oxidation or reduction of the substance which reacts to produce light. We determined the amount of glucose by its reaction to glucose oxidase (GOX) on the surface of the proposed modified electrode, which results hydrogen peroxide (H2O2) as side product. After that the reactions between luminol and H2O2 under oxidizing conditions generate dependent light which can be used to analyze. In the current article at first we proposed a convenient method to obtaining a self-assembly modified electrode. A nano based modified glassy carbon (GC) electrode (Glucose oxidase/Ag nanoparticles/cysteamine (CA)/p-aminobenzene sulfonic acid/GC electrode) was prepared, and the ECL behavior of luminol in the presence of glucose was examined. Compared to the bare GC electrode, the modified electrode incorporating glucose oxidase significantly enhanced the response of the ECL biosensor to glucose due to the enhanced specificity of the modified surface to enzymatic reaction, and the sensitivity of the luminol ECL reaction. Under optimal conditions, the electrode was established to respond linearly to glucose in the concentration range 5.0×10^(-7) to 8.0×10^(-3) mol/L, and the detection limit was established to be a glucose concentration of 4.0×10^(-8)mol/L.
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References
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Chu HH, Guo WY, Di JW, Wu Y, Tu YF, Electroanalysis., 21, 1630 (2009)
Huang JS, Wang DW, Hou HQ, You TY, Adv. Funct. Mater., 18(3), 441 (2008)
