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Received August 18, 2011
Accepted November 2, 2011
articles This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Antioxidant and antibacterial behavior for sediment removed ethanol extract from sea buckthorn seed

1Research Institute of Biotechnology, Dongguk University, 26, Pildong3-ga, Jung-gu, Seoul 100-715, Korea 2Department of Chemical and Biochemical Engineering, Dongguk University, 26, Pildong3-ga, Jung-gu, Seoul 100-715, Korea 3Department of Biological and Environmental Science, Dongguk University, 26, Pildong3-ga, Jung-gu, Seoul 100-715, Korea 4Research Institute of Biotechnology, Department of Chemical and Biochemical Engineering, Dongguk University, 26, Pildong3-ga, Jung-gu, Seoul 100-715, Korea 5Department of Medical Biotechnology/Chemical and Biochemical Engineering, Dongguk University, 26, Pildong3-ga, Jung-gu, Seoul 100-715, Korea
jkpark@dongguk.edu
Korean Journal of Chemical Engineering, August 2012, 29(8), 1069-1073(5), 10.1007/s11814-011-0279-y
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Abstract

The purpose of this study was to develop the separation process removing sediment in sea buckthorn seed ethanol extract, and to investigate the antioxidant and antibacterial behavior for the sediment removed ethanol extract (SBS extract). Sediment such as low solubility materials and oil component was simply removed by filtering after storing the crude extract containing celite for 16 h in a refrigerator at 4 ℃. The SBS extract displayed strong antioxidant activity in the stability test as a function of both storage time and temperature when compared to vitamin C and BHA (Butylated hydroxyanisole) as typical antioxidant ingredients. The bactericidal ratio of the SBS extract against E. coli increased with an increase in the addition of the test agent, and this was particularly significant at concentrations greater than 1,000 μg/ml addition. The bactericidal ratio of 1,000 μg/ml SBS extract against B. subtilis, S. aureus, P. aerusinosa and E. coli was 96.4, 98.6, 91.5, and 52.2% after 2 h, and 98.2, 99.7, 99.3, and 82.6% after 4 h, respectively. These_x000D_ results suggest that the SBS extract may be used as a functional food, pharmaceutical, and cosmetic agent.

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