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- In relation to this article, we declare that there is no conflict of interest.
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Received April 24, 2012
Accepted June 30, 2012
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Copyright © KIChE. All rights reserved.
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Colorimetric detection of vesicle rupture by attack of Ag nanoparticles
Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Korea
korea1@kw.ac.kr
Korean Journal of Chemical Engineering, January 2013, 30(1), 235-237(3), 10.1007/s11814-012-0102-4
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Abstract
Generally, the cytotoxicity effects of nanomaterials on bio-organisms have been examined using live cells or mice by in-vivo or in-vitro testing. These methodologies, however, are time-consuming, and cell-membrane responses for cytotoxicity are often slow. Therefore, in this study, we proposed a simple testing method for colorimetric detection of dye-containing vesicles as biomimetic cell-membranes. When dye-containing vesicles are ruptured by attack of AgNPs_x000D_
(silver nanoparticles), dye in vesicles is released into the solution, and then the color of solution changes to that of dye. Because the ruptured vesicle causes an optical variation, we could easily and quickly monitor the vesicle rupture by AgNPs. And further, SPR (surface plasmon resonance spectroscopy) and TEM (transmission electron microscopy) analysis were carried out to confirm the vesicle rupturing. It will be provide indirect information for cytotoxicity of nanoparticles.
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