An effective process was developed to separate 2,3-butanediol (2,3-BD) from fermentation broth (FB) by reactive-extraction. Propionaldehyde (PRA) was used as reactant and reaction product 2-ethyl-4,5-dimethyl-1,3-dioxolane (EDD) acted as extractant. HCl was selected as catalyst. Appropriate conditions were obtained by experiment as follows: 10 ℃, CHCl=0.2mol·L^(-1), two-stage cross-current extraction, reactant volume ratio (VPRA : VFB) for first stage and second stage is 0.10 and 0.05, respectively. The yield rate of 2,3-butanediol for the whole process can reach 90% w/w, and 2,3-butanediol in the final product can be more than 99% w/w. The novel process required less solution and especially had advantages in treating dilute fermentation broth. Furthermore, equilibrium and kinetic study were investigated on the reaction of propionaldehyde and 2,3-butanediol to provide basic data for process development. The results_x000D_ reveal that reaction enthalpy and activation energy of the reaction were -21.84±2.38 KJ·mol^(-1) and 51.97±2.84 KJ·mol^(-1), respectively. Kinetics was well described by pseudo-homogeneous model.

2,3-Butanediol Reactive-extraction Kinetics Propionaldehyde Fermentation Broth

John W, Knowlton N, J. Amer. Chem. Soc., 68, 208 (1946)
Flickinger MC, Biotechnol. Bioeng., 22, 27 (1980)
Jansen NB, Flickinger MC, Tsao GT, Biotechnol. Bioeng., 26, 362 (1984)
Yu EKC, Levitin N, Biotechnol. Lett., 4, 741 (1982)
Cheng KK, Liu Q, Zhang JA, Process Biochem., 45, 613 (2010)
GARG SK, JAIN A, Bioresour. Technol., 51(2-3), 103 (1995)
Ji XJ, Huang H, Ouyang PK, Biotechnol. Adv., 29, 351 (2011)
Biebl H, Zeng AP, Menzel K, Deckwer WD, Appl. Microbiol. Biotechnol., 50(1), 24 (1998)
Nigam PS, Singh A, Progress in Energy Combust. Sci., 37, 52 (2010)
Wheat JA, Leslie JD, Tomkins RV, Can. J. Res., 26F, 469 (1948)
Shao P, Kumar A, J. Membr. Sci., 329(1-2), 160 (2009)
Li ZG, Teng H, Xiu ZL, Process Biochem., 45, 731 (2010)
Senkus M, Ind. Eng. Chem., 38, 913 (1946)
Xiang BT, Chen SF, Liu DH, J. Tsinghua University., 41, 53 (2001)
Xiu ZL, Zeng AP, Appl. Microbiol. Biotechnol., 6, 917 (2008)
Hao J, Xu F, Liu HJ, Liu DH, J. Chem. Technol. Biotechnol., 81(1), 102 (2006)
Li YJ, Wu YY, Zhu JW, Liu JX, Biotechnol. Biopro. Eng., 17, 317 (2012)
Xie ZM, Zhang WM, Zhang LM, Basic Chemistry, Science Press, Beijing (2009)
Zhang L, Xiu ZM, Yuan XQ, Elements of chemical reaction engineering, East China University of Science and Technology Press, Shanghai (2000)
Hu Y, Lv RD, Liu GJ, Hei EC, Physical Chemistry, Higher Education Press, Beijing (2007)
Zhou J, Bi W, Row KH, Korean J. Chem. Eng., 28(7), 1608 (2011)
Wu B, Zhu J, Chen K, Ji L, Guo J, Zhao J, Korean J. Chem. Eng., 20(2), 354 (2003)
Wang XP, Xing SL, Tianjin Chem. Ind., 23(3), 40 (2009)