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Received April 15, 2012
Accepted August 27, 2012
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Isolation and characterization of autoflocculating mutants of cyanobacterium Arthrospira platensis
1Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University, Busan 617-736, Korea 2Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University, Busan 617-736, Korea 3Korea Food Research Institute, Bundang-gu, Sungnam-si, Gyeonggi-do 463-746, Korea
jhalee@silla.ac.kr
Korean Journal of Chemical Engineering, February 2013, 30(2), 413-416(4), 10.1007/s11814-012-0146-5
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Abstract
Harvesting microalgae is a major concern for mass culture in industry. Flocculation is an easy and effective way to harvest microalgae. However, flocculation using chemical flocculants is not feasible for scaling-up due to their toxicity. As an alternative technique, mutation breeding of autoflocculating microalgae strain has been reported in this study. We characterized autoflocculating mutants of Arthrospira platensis (A. platensis) by ethyl methane sulfonate_x000D_
(EMS). The cells of mutants were aggregated during the culture and dry cell weight increased 1.2- to 1.8-fold compared to the wild type. Autoflocculation was induced highly at an optimal pH level of 9 and the flocculation efficiency reached almost 90%. Mutants showed higher flocculation efficiency irrespective of the addition of chemical flocculants. Thus, it is definitely useful to harvest microalgae using autoflocculating mutants in large-scale culture without any drawbacks_x000D_
of harvested algal biomass.
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Kim SJ, Choi A, Ahn CY, Park CS, Park YH, Oh HM, Lett. Appl. Microbiol., 40, 190 (2005)
