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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received June 18, 2012
Accepted August 12, 2012

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Surface plasmon resonance study of (positive, neutral, negative) vesicles rupture by AgNPs’ attack for screening of cytotoxicity induced by nanoparticles

Ha Nee Umh Younghun Kim^†

Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Korea

korea1@kw.ac.kr

Korean Journal of Chemical Engineering, February 2013, 30(2), 482-487(6), 10.1007/s11814-012-0131-z

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Abstract

As the use of nanomaterials in industrial and commercial applications is growing, official reports concerning possible environmental and health effects of nanoparticles are also steadily increasing. Many toxicological studies on the adverse effects of silver nanoparticles (AgNPs) have used living organisms, which is a time consuming process. Therefore, we propose an alternative method to assess the in-vivo and in-vitro cytotoxicity of nanomaterials, involving a_x000D_ fast and simple screening procedure for vesicle rupture or fusion by the attack of AgNPs. With the assumption that particle interaction between AgNPs and vesicles is induced by electrostatic repulsion or attraction of surface charge, three vesicles with different charges (positive, neutral, and negative) were prepared and they were dispersed with AgNPs in different pH (3, 7, and 10) solutions to control the surface charge of AgNPs. Based on the results of vesicle rupture analyzed by SPR and TEM, screening of cell rupture through vesicles by AgNPs’ attack is determined to be most suitable at pH 7.

Keywords

Silver Nanoparticles Vesicles Nanotoxicity Cell Rupture SPR

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