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Received July 18, 2012
Accepted October 2, 2012
- This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Dispersion stability of citrate- and PVP-AgNPs in biological media for cytotoxicity test
Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Korea 1School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea
korea1@kw.ac.kr
Korean Journal of Chemical Engineering, March 2013, 30(3), 671-674(4), 10.1007/s11814-012-0172-3
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Abstract
Silver nanoparticles (AgNPs) with antimicrobial property have been used for years in various applications, such as antibacterial coating and air/water purification to eliminate microorganisms. In recent years, a number of studies on the potential risk of nanomaterials in humans and the environment have appeared, and thus a large number of studies regarding the toxic effects of AgNPs on cells and micro-organisms have been reported. In in-vivo and invitro cytotoxicity tests, various biological media are used, and thus the dispersion stability of AgNPs in media is important to a successful toxicity test. Therefore, we investigated the dispersion stability of AgNPs in various biological media; PBS (phosphate buffered saline), FBS (fetal bovine serum), and de-ionized water (pH 2, 7, and 9) with and without photo-irradiation. Herein, citrate and PVP (polyvinylpyrrolidone) stabilized AgNPs were used as target materials. In short- and long-term stability tests, we found that PVP-AgNPs stabilized by steric hindrance have good dispersion_x000D_
stability in biological media, compared to citrate-AgNPs stabilized by electrostatic repulsion.
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Krewski D, Andersen ME, Mantus E, Zeise L, Risk Anal., 29, 474 (2009)
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Kim SN, Roh J, Kang MS, Han YA, Lee BS, Kim Y, Park K, Choi K, Park EJ, Environ. Health Toxicol., 25, 215 (2010)
Park EJ, Roh J, Kim Y, Park K, Toxicol. Res., 26, 267 (2010)
Bae E, Park HJ, Yoon J, Kim Y, Choi K, Yi J, Korean J. Chem. Eng., 28(1), 267 (2011)
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