Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 18, 2012
Accepted October 2, 2012

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Dispersion stability of citrate- and PVP-AgNPs in biological media for cytotoxicity test

Jinkyu Roh Ha Nee Umh Jaehoon Sim Sumin Park¹ Jongheop Yi¹ Younghun Kim^†

Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Korea ¹School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea

korea1@kw.ac.kr

Korean Journal of Chemical Engineering, March 2013, 30(3), 671-674(4), 10.1007/s11814-012-0172-3

Download PDF

Abstract

Silver nanoparticles (AgNPs) with antimicrobial property have been used for years in various applications, such as antibacterial coating and air/water purification to eliminate microorganisms. In recent years, a number of studies on the potential risk of nanomaterials in humans and the environment have appeared, and thus a large number of studies regarding the toxic effects of AgNPs on cells and micro-organisms have been reported. In in-vivo and invitro cytotoxicity tests, various biological media are used, and thus the dispersion stability of AgNPs in media is important to a successful toxicity test. Therefore, we investigated the dispersion stability of AgNPs in various biological media; PBS (phosphate buffered saline), FBS (fetal bovine serum), and de-ionized water (pH 2, 7, and 9) with and without photo-irradiation. Herein, citrate and PVP (polyvinylpyrrolidone) stabilized AgNPs were used as target materials. In short- and long-term stability tests, we found that PVP-AgNPs stabilized by steric hindrance have good dispersion_x000D_ stability in biological media, compared to citrate-AgNPs stabilized by electrostatic repulsion.

Keywords

Silver Nanoparticles Cytotoxicity Dispersion Stability Citrate PVP

References

Shipway AS, Katz E, Willner I, Chemphyschem., 1, 18 (2000)
Sharma VK, Yngard RA, Lin Y, Adv. Colloid Interface Sci., 145, 83 (2009)
Seo WS, Kim TT, Sung JS, Song KC, Korean Chem. Eng. Res., 42(1), 78 (2004)
Choi WI, Yu DG, Yang MC, Polym. Adv. Technol., 16, 600 (2005)
Park E, Roh J, Kim Y, Choi K, J. Health Sci., 57, 60 (2011)
Bae E, Park HJ, Lee J, Kim Y, Yoon J, Park K, Choi K, Yi J, Environ. Toxicol. Chem., 29, 2154 (2010)
Krewski D, Andersen ME, Mantus E, Zeise L, Risk Anal., 29, 474 (2009)
Roh J, Park EJ, Park K, Yi J, Kim Y, Korean J. Chem. Eng., 27(6), 1897 (2010)
Kim SN, Roh J, Kang MS, Han YA, Lee BS, Kim Y, Park K, Choi K, Park EJ, Environ. Health Toxicol., 25, 215 (2010)
Park EJ, Roh J, Kim Y, Park K, Toxicol. Res., 26, 267 (2010)
Bae E, Park HJ, Yoon J, Kim Y, Choi K, Yi J, Korean J. Chem. Eng., 28(1), 267 (2011)
Cedervall T, Lynch I, Lindman S, Berggard T, Thulin E, Nilsson H, Dawson KA, Linse S, P. Natl. Acad. Sci., 104, 2050 (2007)
OECD WPMN, Dossier develoment plan: Silver nanoparticles, ENV/CHEM/NANO(2009)4/ADD4.
Wang HS, Qiao XL, Chen JG, Wang XJ, Ding SY, Mater. Chem. Phys., 94(2-3), 449 (2005)
Zhou Y, Han ST, Xu ZX, Roy VAL, J. Mater. Chem., 22, 4060 (2012)
Kim Y, Kim C, Choi I, Rengaraj S, Yi J, Environ. Sci. Technol., 38, 924 (2004)
Redmond PL, Wu X, Brus L, J. Phys. Chem. C., 111, 8942 (2007)
Wu XM, Redmond PL, Liu HT, Chen YH, Steigerwald M, Brus L, J. Am. Chem. Soc., 130(29), 9500 (2008)
Xue C, Metraux GS, Millstone JE, Mirkin CA, J. Am. Chem. Soc., 130(26), 8337 (2008)