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Received July 4, 2012
Accepted January 23, 2013
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Simulation of computational fluid dynamics and comparison of cephalosporin C fermentation performance with different impeller combinations
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China 1CSPC Hebei Zhongrun Pharmaceutical Co., Ltd., Shijiazhuang, Hebei 050041, China
Korean Journal of Chemical Engineering, May 2013, 30(5), 1097-1104(8), 10.1007/s11814-013-0010-2
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Abstract
Cephalosporin C (CPC) fermentation by Acremonium chrysogenum is an extremely high oxygen-consuming process and oxygen transfer rate in a bioreactor directly affects fermentation performance. In this study, fluid dynamics and oxygen transfer in a 7 L bioreactor with different impellers combinations were simulated by computational fluid dynamics (CFD) model. Based on the simulation results, two impeller combinations with higher oxygen transfer rate (KLa) were selected to conduct CPC fermentations, aiming at achieving high CPC concentration and low_x000D_
accumulation of major by-product, deacetoxycephalosporin (DAOC). It was found that an impeller combination with a higher KLa and moderate shear force is the prerequisite for efficient CPC production in a stirred bioreactor. The best impeller combination, which installed a six-bladed turbine and a four-pitched-blade turbine at bottom and upper layers but with a shortened impellers inter-distance, produced the highest CPC concentration of 35.77 g/L and lowest DAOC/_x000D_
CPC ratio of 0.5%.
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Chiang SJ, J. Ind. Microbiol. Biotechnol., 31, 99 (2004)
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Basak S, Velayudhan A, Ladisch MR, Biotechnol. Prog., 11(6), 626 (1995)
