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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received August 17, 2012
Accepted December 16, 2012

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Enhanced production of carboxymethylcellulase by Cellulophaga lytica LBH-14 in pilot-scale bioreactor under optimized conditions involved in dissolved oxygen

Wa Cao^{1 2} Sang-Un Lee^{1 2} Jianhong Li³ Jin-Woo Lee^{2 4†}

¹Department of Medical Bioscience, Graduate School of Dong-A University, Busan 604-714, Korea ²BK21 Bio-Silver Program of Dong-A, Busan 604-714, Korea ³College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, Hebei 430070, China ⁴Department of Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea

jwlee@dau.ac.kr

Korean Journal of Chemical Engineering, May 2013, 30(5), 1105-1110(6), 10.1007/s11814-012-0219-5

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Abstract

The optimal conditions for the production of carboxymethylcellulase (CMCase) by Cellulophaga lytica LBH-14 at flask scale has been previously reported. In this study, we optimized the parameters involved in dissolved oxygen in 7 and 100 L bioreactors for pilot-scaled production of CMCase by C. lytica LBH-14. The optimal conditions of agitation speed and aeration rate for cell growth in 7 L bioreactors were 395 rpm and 0.98 vvm, whereas those for production of CMCase were 357 rpm and 0.55 vvm. The optimal inner pressures for cell growth and production of CMCase by C. lytica LBH-14 in 100 L bioreactors were 0.00 and 0.06MPa, respectively. The production of CMCase under an optimized inner pressure was 1.38 times higher than that without an inner pressure. The maximal production of CMCase by C. lytica under optimized conditions at pilot scale using rice bran and ammonium chloride was 153.6U/mL, which was 1.39 times higher than that at flask scale.

Keywords

Cellulophaga lytica Carboxymethylcellulase Marine Microorganism Pilot-scaled Production Response Surface Method

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