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Received November 30, 2012
Accepted April 14, 2013
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Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production
Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea 1College of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea 2Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, Seoul 139-701, Korea
kimsw@korea.ac.kr
Korean Journal of Chemical Engineering, June 2013, 30(6), 1335-1338(4), 10.1007/s11814-013-0058-z
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Abstract
Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.
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