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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received December 27, 2012
Accepted June 17, 2013

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A three-zone simulated moving-bed for separation of immunoglobulin Y

Sung-Moon Song In Ho Kim^†

Department of Chemical Engineering, Chungnam National University, 220, Gung-dong, Yuseong-gu, Daejeon 305-764, Korea

ihkim@cnu.ac.kr

Korean Journal of Chemical Engineering, August 2013, 30(8), 1527-1532(6), 10.1007/s11814-013-0106-8

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Abstract

Separation of immunoglobulin Y (IgY) from hen eggs is an interesting topic for investigating antibody purification. Understanding of IgY separation by chromatography is necessary to prepare antibody. IgY preparation in SMB was simulated by Aspen chromatography and was experimented by assembling a 3-zone simulated moving bed (SMB). With change of stream flow rates, the SMB performance was studied. Simulation of IgY batch HPLC chromatography was also done and confirmed by HPLC experiments. Based on these, good operating conditions of SMB chromatography were determined. Three-zone SMB equipment was set up by connecting three C18-HPLC columns, four HPLC pumps, and six multiposition valves. Batch chromatography of IgY was conducted to determine the isotherms of IgY and contaminating impurity. The outlet streams of SMB, raffinate and extract were sampled and analyzed by analytical HPLC system. The adsorption isotherms of IgY and impurity were determined as HIgY=0.1 and Himpure=1.1. The highest experimental purity of IgY from SMB was obtained as 98% for the operating parameters of Qfeed=0.2mL/min, Qdesorbent=0.31 mL/min, Qextract=0.2 mL/min, Qraffinate=0.3 mL/min, and switching time=8.47 min.

Keywords

IgY SMB (Simulated Moving Bed)

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