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Received January 22, 2013
Accepted May 30, 2013
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Growth kinetics of an indigenous mixed microbial consortium during methylene chloride degradation in a batch reactor
Department of Chemical Engineering, Annamalai University, Chidambaram, Tamil Nadu-608001, India 1Department of Civil Engineering, Indian Institute of Technology, Madras, India 2Department of Chemical Engineering, Indian Institute of Technology, Madras, India
Korean Journal of Chemical Engineering, September 2013, 30(9), 1770-1774(5), 10.1007/s11814-013-0099-3
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Abstract
Biodegradation of methylene chloride by a mixed microbial culture, isolated from a common sewage treatment plant, was investigated in a batch system. Batch experiments were performed at room temperature (27 ℃) and pH value of 7. The methylene chloride concentration in growth media varied from 25 mgl^(-1) to 250mgl^(-1). A maximum observed degradation was 1 mgl.1h.1 at 100 mgl.1 of methylene chloride. The culture followed substrate inhibition kinetics and specific growth rate were fitted to different substrate inhibition models (Haldane, Aiba and Edwards models) by MATLAB 7.1@. Among all models, Haldane was found to better fit with root mean square of 0.947. The biokinetic constants estimated using these models show good potential of the mixed microbial culture in methylene Chloride degradation. Escherichia coli and Staphylococcus aureus are predominant microbes present in the mixed culture.
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Trotsenko YA, Doronina NV, Tourova TP, Kuznetzov BB, Leisinger T, Systematic and Appl. Microbiol., 23, 210 (2000)
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Gokulakrishanan S, Sathyanarayana Gummadi N, Process Biochem., 41(6), 1417 (2006)
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