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Received April 4, 2013
Accepted June 17, 2013
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Anti-inflammatory effects of silkworm hemolymph on lipopolysaccharide-stimulated macrophages
Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, Doryong-dong, Yuseong-gu, Daejeon 305-340, Korea 1Department of Dermatology, Seoul National University College of Medicine, 28, Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea 2Division of Bioengineering, Incheon National University, 119, Academy-ro, Yeonsu-gu, Incheon 406-772, Korea 3School of Chemical and Biological Engineering, Bio-MAX Institute, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul 151-744, Korea
thpark@snu.ac.kr, ekim@kistep.re.kr
Korean Journal of Chemical Engineering, September 2013, 30(9), 1784-1789(6), 10.1007/s11814-013-0108-6
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Abstract
Macrophages participate in several inflammatory pathologies such as sepsis and arthritis. We investigated the effect of silkworm hemolymph (SH) on the LPS-induced pro-inflammatory macrophages. SH inhibits LPSinduced nitric oxide (NO) production in RAW 264.7 cells and murine peritoneal macrophages. The decreased NO was reflected as a decreased amount of inducible nitric oxide synthase (iNOS) mRNA and protein. It was also found that SH inhibited pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α production. To elucidate the mechanism by which SH inhibits NO production and iNOS expression, we investigated that SH suppressed IκB phosphorylation, which leads to the activation of NF-κB followed by degradation of IκB. This observation suggests that SH is a potential therapeutic modulator for inflammation-associated disorders.
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References
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Choi SS, Rhee WJ, Kim EJ, Park TH, Biotechnol. Bioeng., 95(3), 459 (2006)
Wang Z, Park JH, Park HH, Tan WS, Park TH, Biotechnol. Bioeng., 108(7), 1634 (2011)
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Joosten CE, Park TH, Shuler ML, Biotechnol. Bioeng., 83(6), 695 (2003)
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Rhee WJ, Lee EH, Park JH, Lee JE, Park TH, Biotechnol. Prog., 23(6), 1441 (2007)
D’Acquisto F, May MJ, Ghosh S, Mol. Interv., 2, 22 (2002)
Starnes HF, Pearce MK, Tewari A, Yim JH, Zou JC, Abrams JS, J. Immunol., 145, 4185 (1990)
Rollins BJ, Mol. Med. Today., 2, 198 (1996)
Van Kaer L, Immunol. Cell Biol., 82, 315 (2004)
Ghosh S, May MJ, Kopp EB, Annu. Rev. Immunol., 16, 225 (1998)
Xaus J, Comalada M, Valledor AF, Lioberas J, Lopez-Soriano F, Argiles JM, Bogdan C, Celada A, Blood., 15, 3823 (2000)
Koizumi N, Morozumi A, Imamura M, Tanaka E, Iwahana H, Sato R, Eur. J. Biochem., 248, 217 (1997)
Koizumi N, Imamura M, Kadotani T, Yaoi K, Iwahana H, Sato R, Febs Lett., 443, 139 (1999)
Kamimura M, Nakahara Y, Kanamori Y, Tsuzuki S, Hayakawa Y, Kiuchi M, Biochem. Biophys. Res. Commun., 286(1), 67 (2001)
Ishii K, Hamamoto H, Kamimura M, Nakamura Y, Noda H, Imamura K, Mita K, Sekimizu K, J. Biol. Chem., 285, 28635 (2010)
