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Received November 23, 2013
Accepted March 14, 2014
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Optimization of medium components using orthogonal arrays for γ-Linolenic acid production by Spirulina platensis
Srinivasa Reddy Ronda†
Pavani Lakhsmi Chandrika Parupudi
Sandeep Vemula
Santhosh Tumma
Mahendran Botlagunta
Vijaya Saradhi Settaluri
Smita Lele1
Suraj Sharma2
Chari Kandala3
Department of Biotechnology, KL University, Green Fields, Vaddeswaram, Guntur-522502, Andhra Pradesh, India 1Food Engineering and Technology Department, Institute of Chemical Technology, N ParekhMarg, Matunga, Mumbai-40019, India 2Department of Textiles, Merchandising and Interiors, College of Family and Consumer Sciences, The University of Georgia, Athens, GA 30602, USA 3United States Department of Agriculture, Dawson, USA
Korean Journal of Chemical Engineering, October 2014, 31(10), 1839-1844(6), 10.1007/s11814-014-0082-7
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Abstract
This work describes the medium optimization of γ-Linolenic acid (GLA) production by Spirulina platensis using one-factor and orthogonal array design methods. In the one-factor experiments, NaHCO3 (9 mg L^(-1)), NaNO3 (13.5 mg L^(-1)) and MgSO4ㆍ7H2O (11.85 mg L^(-1)) proved to be the best components for GLA production. The optimal pH for GLA production by the alga was 9.2. Based on the delta values, NaHCO3 showed the greatest effect on the GLA production of the various factors tested, followed in decreasing order by MgSO4ㆍ7H2O, NaNO3 and K2SO4. The maximum GLA yield obtained was 19.2 mgL^(-1) in the presence of optimum concentrations of NaHCO3 (20 g L^(-1)), NaNO3 (3 g L^(-1)), MgSO4ㆍ7H2O (0.5 g L^(-1)) and K2SO4 (1.5 g L^(-1)). Because of the slow growth rate of the algae, the practice of robust orthogonal array methods during the optimization of medium components can result in the production of an optimal biomass and a higher GLA yield for nutraceutical applications.
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Xu CP, Kim SW, Hwang HJ, Choi JW, Yun JW, Process. Biochem., 38, 1025 (2003)
Daneshvar N, Khataee AR, Rasoulifard MH, Pourhassan M, J. Hazard. Mater., 143(1-2), 214 (2007)
De BK, Chaudhury S, Bhattacharyya DK, J. Am. Oil Chem. Soc., 76, 153 (1999)
Vonshak A, Abeliovich A, Boussiba S, Arad S, Richmond A, Biomass, 2, 175 (1982)
Fagiri YMA, Salleh A, El-Nagerabi SAF, Afr. J. Biotechnol., 12, 5458 (2013)
Kim K, Hoh D, Ji Y, Do H, Lee B, Holzapfel W, Biomass Bioenerg., 49, 181 (2013)
Ogbonda KH, Aminigo RE, Abu GO, Bioresour. Technol., 98(11), 2207 (2007)
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