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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received August 30, 2013
Accepted January 23, 2014

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of shear stress on the growth of continuous culture of Synechocystis PCC 6803 in a flat-panel photobioreactor

Min-Gyu Sung¹ Won-Sub Shin¹ Woong Kim¹ Jong-Hee Kwon^{1 2†} Ji-Won Yang^{1 3†}

¹Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Korea ²Plant Biochemistry, Faculty of Biology & Biotechnology, Ruhr University Bochum, D-44780 Bochum, Germany ³Advanced Biomass R&D Center, KAIST, Yuseong-gu, Daejeon 305-701, Korea

jhkwon75@kaist.ac.kr

Korean Journal of Chemical Engineering, July 2014, 31(7), 1233-1236(4), 10.1007/s11814-014-0029-z

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Abstract

The effect of hydrodynamic forces generated by air bubbles on cell growth of continuous culture of Synechocystis PCC 6803 was studied in a flat-panel photobioreactor. Keeping all relevant parameters constant enables the optimization of individual parameters, for which a continuous cultivation approach has significant advantages. Continuous culture of Synechocystis PCC 6803 was cultivated under different gas velocities from 0.022 m s^(-1) up to 0.128 m s^(-1). Based on direct determination of effective growth rate at constant cell densities, cell damage due to shear stress induced by the increasing gas velocity at the sparger was directly observed. A significant decrease of effective growth rate was observed at gas velocity of 0.085 m s^(-1) generated at the gas flow rate of 200 ml min^(-1), indicating cell damage by shear stress. Optimization of gas volume and the development of an effective aeration system corresponding to a given reactor setup is important to realize a reliable cell growth.

Keywords

Shear Stress Bioprocess Optimization Gas Flow Rate Continuous Cultivation Photobioreactor

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