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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received November 28, 2013
Accepted February 21, 2014

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Improvement of fermentative production of exopolysaccharides from Aureobasidium pullulans under various conditions

Charles Seo Hong Won Lee¹ Arumuganainar Suresh Ji Won Yang Jun Ki Jung¹ Yeu-Chun Kim^†

Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 305-701, Korea ¹Biotechnology Process Engineering Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea

Korean Journal of Chemical Engineering, August 2014, 31(8), 1433-1437(5), 10.1007/s11814-014-0064-9

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Abstract

The optimization of exopolysaccharide (EPS) production was investigated in the polymorphic fungal strain of Aureobasidium pullulans (KCTC 6081) with varying pH, nutrients concentration, and mixing parameters in batch fermentation condition. The maximum production of EPS (~7.5 g/L) was observed at pH 4, while optimum nutrient concentration of carbon (sucrose), nitrogen (NaNO3), phosphorous (K2HPO4), and ascorbic acid was 50 g/L, 5 g/L, 1 g/L and 2 g/L, respectively. Interestingly, EPS productivity under non pH controlled fermentation conditions was_x000D_ 0.12 g/L/h with 400 rpm mixing, while under a controlled pH of 4, the EPS productivity was 0.21 g/L/h with 600 rpm, respectively. The fed-batch fermentation increased the EPS productivity up to 0.345 g/L/h with changing mixing conditions from 200 to 600 rpm and reached 47 g/L with 88% pullulan. Thus, pH and mixing were the key parameters for enhancing EPS production from A. pullulans. It is expected that these optimized parameters can be well used for enhanced industrial production of pullulan.

Keywords

Optimization Fermentation Exopolysaccharide Agitation Nutrients

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