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Received December 23, 2014
Accepted April 18, 2015
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Optimization of lipid extraction from marine green macro-algae as biofuel resources
Department of Biotechnology, Pukyong National University, Busan 608-737, Korea 1Department of Biotechnology and Bioengineering, Interdisciplinary Program of Graduate, School for Bioenergy and Biomaterials, Chonnam National University, Gwangju 500-757, Korea
gtjeong@pknu.ac.kr
Korean Journal of Chemical Engineering, December 2015, 32(12), 2463-2467(5), 10.1007/s11814-015-0083-1
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Abstract
The extraction of lipid from Enteromorpha intestinalis was investigated. Among 13 types of organic solvent, the chloroform/methanol (2 : 1, v/v) system obtained the highest content. Considering the lipid extraction procedure and extracted amount, the optimal solid/liquid ratio was 1 : 50 (w/v). With increasing extraction temperature, the content was enhanced until 45 oC, after which it increased only slightly. Correspondingly, with increasing extraction time, the content linearly increased until 2.5 hr, after which it held steady. From a fatty acid compositional analysis of the lipid extracted from E. intestinalis, the content of palmitic acid and stearic acid was 39.85±1.19% and 14.61±0.80%, respectively. The unsaturated fatty acids (C18:1, C18:2 and C18:3) concentrations, meanwhile, were 0.3-3.2%.
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Suganya T, Renganathan S, Bioresour. Technol., 107, 319 (2012)
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