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Received November 26, 2015
Accepted June 20, 2016
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Potential industrial application of Actinobacillus succinogenes NJ113 for pyruvic acid production by microaerobic fermentation
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, P. R. China
kqchen@njtech.edu.cn
Korean Journal of Chemical Engineering, October 2016, 33(10), 2908-2914(7), 10.1007/s11814-016-0168-5
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Abstract
Actinobacillus succinogenes NJ113 is capable of microaerobic fermentation, which offers the possibility of a novel type of pyruvic acid production. A dissolved oxygen environment with stirring at 300 rpm was a key factor in the fermentative production of a maximum concentration of pyruvic acid. Potassium carbonate (K2CO3) was found to have a role in promoting pyruvic acid production, influencing the concentration of pyruvic acid and production of the by-product succinic acid. The final titer of pyruvic acid production was 36.8±0.1 g L-1 with an overall yield of_x000D_
0.639±0.056 g g-1 glucose and 3.12±0.03mmol g-1 dry cell weight h-1. Significance and impact of the study: This study is the first to illustrate the advantage of using Actinobacillus succinogenes NJ113 with no genetic modification under microaerobic conditions for the production of pyruvic acid.
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References
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Izumi Y, Matsumura Y, Tani Y, Yamada H, Agric. Biol. Chem., 46, 2673 (1982)
Yanase H, Mori N, Masuda M, Kita K, Shimao M, Kato N, J. Ferment. Bioeng., 73, 287 (1992)
