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Received July 24, 2015
Accepted October 1, 2015
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Engineering a chimeric malate two-component system by introducing a positive feedback loop in Escherichia coli
Department of Chemical Engineering, University of Ulsan, Ulsan 44610, Korea
shhong@ulsan.ac.kr
Korean Journal of Chemical Engineering, March 2016, 33(3), 972-975(4), 10.1007/s11814-015-0209-5
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Abstract
Previous studies constructed a chimeric MalKZ two-component system to sense environmental malate. In this study, we used a positive feedback loop to accelerate and amplify the output signal indicating malate concentration. The positive feedback loop was constructed by cloning ompR gene, which encodes ompC and induces OmpR protein; ompC promoter was used to control the process. The transcriptional expression profile showed that the expression level of ompC gene increased about two-fold after the positive feedback loop was introduced. When GFP was used as a reporter protein, a 71% increase in fluorescence level was observed. The results indicate that the signal transduction kinetics of MalKZ can be engineered by introducing the positive feedback loop.
References
Domb AJ, Laurencin CT, Israeli O, Gerhart TN, Langer R, J. Polym. Sci. A: Polym. Chem., 28, 973 (1990)
Jacvic M, Dunjic B, Djonlagic J, Spassky N, Sepulchre M, Sepulchre MO, Polym. Bull., 28, 621 (1992)
Lee SY, Hong SH, Lee SH, Park SJ, Macromol. Biosci., 4, 157 (2004)
Nguyen T, Hong S, Biotechnol. Bioeng., 13, 288 (2008)
Stock AM, Robinson VL, Goudreau PN, Annu. Rev. Biochem., 69, 215 (2000)
Laub MT, Goulian M, Annu. Rev. Gene., 41, 121 (2007)
Ganesh I, Ravikumar S, Yoo IK, Hong S, Bioprocess Biosyst Eng., 38, 797 (2015)
Chang DE, Leung S, Atkinson MR, Reifler A, Forger D, Ninfa AJ, Proc. Natl. Acad. Sci. U.S.A., 107, 175 (2010)
Thattai M, Van Oudenaarden A, Proc. Natl. Acad. Sci. U.S.A., 98, 8614 (2001)
Pham V, Ravikumar S, Lee S, Hong S, Yoo IK, Bioprocess Biosyst Eng., 36, 1185 (2013)
Ravikumar S, Pham V, Lee S, Yoo IK, Hong S, J. Ind. Microbiol. Biotechnol., 39, 861 (2012)
Yim SS, Choi JW, Lee RJ, Lee YJ, Lee SH, Kim SY, Jeong KJ, Biotechnol. Bioeng., 9999, 1 (2015)
Eleaume H, Jabbouri S, J. Microbiol. Methods, 59, 363 (2004)
Yim SS, An SJ, Kang M, Lee J, Jeong KJ, Biotechnol. Bioeng., 110(11), 2959 (2013)
Ravikumar S, Ganesh I, Maruthamuthu M, Hong S, Korean J. Chem. Eng., In Press (2015)
Jacvic M, Dunjic B, Djonlagic J, Spassky N, Sepulchre M, Sepulchre MO, Polym. Bull., 28, 621 (1992)
Lee SY, Hong SH, Lee SH, Park SJ, Macromol. Biosci., 4, 157 (2004)
Nguyen T, Hong S, Biotechnol. Bioeng., 13, 288 (2008)
Stock AM, Robinson VL, Goudreau PN, Annu. Rev. Biochem., 69, 215 (2000)
Laub MT, Goulian M, Annu. Rev. Gene., 41, 121 (2007)
Ganesh I, Ravikumar S, Yoo IK, Hong S, Bioprocess Biosyst Eng., 38, 797 (2015)
Chang DE, Leung S, Atkinson MR, Reifler A, Forger D, Ninfa AJ, Proc. Natl. Acad. Sci. U.S.A., 107, 175 (2010)
Thattai M, Van Oudenaarden A, Proc. Natl. Acad. Sci. U.S.A., 98, 8614 (2001)
Pham V, Ravikumar S, Lee S, Hong S, Yoo IK, Bioprocess Biosyst Eng., 36, 1185 (2013)
Ravikumar S, Pham V, Lee S, Yoo IK, Hong S, J. Ind. Microbiol. Biotechnol., 39, 861 (2012)
Yim SS, Choi JW, Lee RJ, Lee YJ, Lee SH, Kim SY, Jeong KJ, Biotechnol. Bioeng., 9999, 1 (2015)
Eleaume H, Jabbouri S, J. Microbiol. Methods, 59, 363 (2004)
Yim SS, An SJ, Kang M, Lee J, Jeong KJ, Biotechnol. Bioeng., 110(11), 2959 (2013)
Ravikumar S, Ganesh I, Maruthamuthu M, Hong S, Korean J. Chem. Eng., In Press (2015)
