A laboratory-scale stirred basket reactor (SBR) was constructed to study the synthesis of an n-butyl oleate ester using Novozym 435. An ester yield of approximately 98% was obtained after 6 h using an equimolar substrate ratio, 3.5 g of enzyme, a reaction temperature of 40 oC, and an impeller speed of 200 rpm. The kinetic data were modeled as a ping-pong bi-bi mechanism using a non-linear regression technique. Statistical analysis of the results showed that a model that incorporated the inhibitory effect of n-butanol yielded the best fit with the following parameters: Vmax=24.8mmol L-1 min-1, Km, oleic acid=190.8mM, Km, n-butanol=544.7 mM, and Ki, n-butanol=158.3mM. Mass transfer effects on the enzyme kinetics were also studied, and the absence of internal and external diffusion limitations on the reaction in the SBR was confirmed by considering calculated values of the Thiele modulus and the Damkohler number. Novozym 435 exhibited satisfactory performance in repeated-batch experiments using SBR.

Stirred Basket Reactor Biodiesel Additive Ester Synthesis Immobilized Lipase Kinetic Parameters

Hajar M, Vahabzadeh F, Ind. Crop. Prod., 52, 430 (2014)
Hajar M, Vahabzadeh F, Ind. Crop. Prod., 59, 252 (2014)
Salih N, Salimon J, Yousif E, Ind. Crop. Prod., 34, 1089 (2011)
Salimon J, Salih N, Yousif E, Eur. J. Lipid Sci. Technol., 112, 519 (2010)
Lee JH, Kim SB, Yoo HY, Lee JH, Park C, Han SO, Kim SW, Korean J. Chem. Eng., 30(6), 1272 (2013)
Lee JH, Kim SB, Yoo HY, Lee JH, Han SO, Park C, Kim SW, Korean J. Chem. Eng., 30(6), 1335 (2013)
Ghamgui H, Karra-Chaabouni M, Gargouri Y, Enzyme Microb. Technol., 35(4), 355 (2004)
Balcao VM, Paiva AL, Malcata FX, Enzyme Microb. Technol., 18(6), 392 (1996)
Doran PM, Bioprocess engineering principles, 2nd Ed., Academic Press (2013).
Do DD, Chem. Eng. J., 18, B51 (1984)
Purich DL, Enzyme kinetics: Catalysis control: A reference of theory and best-practice methods, Elsevier (2010).
Lowry RR, Tinsley IJ, J. Am. Oil Chem. Soc., 53, 470 (1976)
Kwon DY, Rhee JS, J. Am. Oil Chem. Soc., 63, 89 (1986)
Lapin LL, Modern engineering statistics, Belmont CA, USA, Wadsworth Publishing Company (1997).
Chaibakhsh N, Abdul Rahman MB, Vahabzadeh F, Abd-Aziz S, Basri M, Salleh AB, Biotechnol. Bioeng., 15, 846 (2010)
Basri M, Kassim MA, Mohamad R, Ariff AB, J. Mol. Catal. B-Enzym., 85-86, 214 (2013)
Hajar M, Shokrollahzadeh S, Vahabzadeh F, Monazzami A, Enzyme Microb. Technol., 45(3), 188 (2009)
Zaidi A, Gainer JL, Carta G, Mrani A, Kadiri T, Belarbi Y, Mir A, J. Biotechnol., 93, 209 (2002)
Yadav GD, Devendran S, Process Biochem., 47, 496 (2012)
Bezbradica D, Mijin D, Siler-Marinkovic S, Knezevic Z, J. Mol. Catal. B-Enzym., 38, 11 (2006)
Suchmacher M, Geller M, Practical biostatistics, Elsevier (2012).
Blanch HW, Clark DS, Biochemical Engineering, Marcel Dekker, New York (1997).
Wilke CR, Chang P, AIChE J., 1, 264 (1955)
Tyn MT, Calus WF, Processing, 21, 16 (1975)
Joback KG, Reid RC, Chem. Eng. Commun., 57, 233 (1987)
Carberry JJ, Chemical and catalytic reaction engineering, McGraw-Hill, New York (1976).
Dong HP, Wang YJ, Zheng YG, J. Mol. Catal. B-Enzym., 66, 90 (2010)
Chulalaksananukul W, Condoret JS, Delorme P, Willemot RM, Febs Lett., 276, 181 (1990)
Durbin J, Watson GS, Biometrika., 38, 159 (1951)
Remero MD, Calvo L, Alba C, Daneshfar A, J. Biotechnol., 127, 269 (2007)
Daneshfar A, Ghaziaskar HS, Shiri L, Manafi MH, Nikorazm M, Abassi S, Biochem. Eng. J., 37, 279 (2007)
Pouchert CJ, Behnke J, The Aldrich Library of 13C and 1H FT NMR Spectra, Aldrich Chemical Co. (1993).
Chowdhury A, Chakraborty R, Mitra D, Biswas D, Ind. Crop. Prod., 52, 783 (2014)