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Received March 30, 2015
Accepted December 8, 2015
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Lipase-catalyzed glycerolysis of olive oil in organic solvent medium:Optimization using response surface methodology
Department of Chemical Engineering, Sardar Vallabhbhai National Institute of Technology, Surat 395007, Gujarat, India
Korean Journal of Chemical Engineering, April 2016, 33(4), 1247-1254(8), 10.1007/s11814-015-0272-y
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Abstract
Enzymatic glycerolysis of olive oil for mono- (MG) and diglycerides (DG) synthesis was investigated. Several pure organic solvents and co-solvent mixtures were screened in a batch reaction system. The yields of MG and DG in co-solvent mixtures exceeded those of the corresponding pure organic solvents. Batch reaction conditions of the glycerolysis reaction, the lipase amount, the glycerol to oil molar ratio, the reaction time, and temperature, were studied. In these systems, the high content of reaction products, especially MG (55.8 wt%) and DG (16.4wt%) was achieved at 40 oC temperature and 0.025 g of lipase with relatively low glycerol to oil molar ratio (2 : 1) within 4 h of reaction time in isopropanol/tert-butanol (1 : 3) solvent mixture. Glycerolysis reaction was optimized with the assistance of response surface methodology (RSM). Optimal condition for reaction conversion was recommended as lipase amount 0.025 g, glycerol to oil molar ratio 2 : 1, reaction time 4 h and temperature 40 ℃.
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References
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Yang T, Rebsdorf M, Engelrud U, Xu X, J. Food Lip., 12, 299 (2005)
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