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Received July 19, 2015
Accepted January 11, 2016
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Bio-electrochemical conversion of atmospheric N2 to ammonium using free-living diazotrophs
Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, Daejeon 34057, Korea 1Department of Physiology, College of Medicine, Hanyang University, Seoul 04763, Korea 2Department of Nano Convergence, Seokyeong University, Seoul 02713, Korea
baakdoo@skuniv.ac.kr
Korean Journal of Chemical Engineering, June 2016, 33(6), 1865-1871(7), 10.1007/s11814-016-0011-z
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Abstract
The effects of electrochemical reducing power on enrichment, growth, and ammonium production of freeliving diazotrophs from rhizosphere soil were evaluated. Soil bacteria were cultivated in a conventional bioreactor (CBR) and an electrochemical bioreactor (EBR), both containing a neutral red-modified graphite felt (NR-GF) cathode and a platinum anode, but with electricity charged to the EBR only. Temperature gradient gel electrophoresis identified 21 species from rhizosphere soil, and 17 and seven species from the CBR and EBR, respectively, after 40 days of incubation. Six species from the CBR and five species from the EBR were diazotrophs. The bacterial community biomass and the ammonium content in the bacterial culture were, respectively, 1.6 and 2 times higher in the EBR than in the CBR. These results indicate that the electrochemical reducing power generated from the NR-GF may be a driving force in the activation of enrichment, growth, and N2-fixing metabolism of diazotrophs.
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Hosseini SM, Hamidi A, Moghadassi A, Madaeni SS, Korean J. Chem. Eng., 32(3), 429 (2015)
Cheung PY, Kinkle BK, Appl. Environ. Microbiol., 67, 2222 (2001)
Orr CH, James A, Leifert C, Cooper JM, Cummings SP, Appl. Environ. Microbiol., 77, 911 (2010)
Yang CH, Crowley DE, Appl. Environ. Microbiol., 66, 345 (2000)
Holl CM, Montoya JP, J. Phycol., 41, 1178 (2005)
Christiansen-Weniger C, van Ven JA, Biol. Fertil. Soils, 12, 100 (1991)
Jeon BY, Jung IL, Park DH, J. Environ. Protect., 3, 55 (2012)
Agawin NAE, Limnol. Oceanogr., 52, 2233 (2007)
Oritiz-Marquez JCF, Nascimento MD, de los Angeles Dublan M, Curatti L, Appl. Environ. Microbiol., 78, 2345 (2012)
Chen GW, Choi SJ, Cha JH, Lee TH, Kim CW, Korean J. Chem. Eng., 27(5), 1513 (2010)
Shanmugam KT, Valentine RC, Proc. Natl. Acad. Sci. USA, 72, 136 (1975)
Colnaghi R, Green A, He L, Rudnick P, Kennedy C, Plant Soil, 194, 145 (1997)
Hongo M, Iwahara M, Agric. Biol. Chem., 43, 2075 (1979)
Gregory KB, Bond DR, Lovely DR, Environ. Microbiol., 6, 596 (2004)
Rabaey K, Girguis P, Nielsen LK, Curr. Opin. Biotechnol., 22, 1 (2011)
