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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received November 2, 2015
Accepted February 9, 2016

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Biocompatibility of cobalt iron oxide magnetic nanoparticles in male rabbits

Tanveer Ahmad Tabish^{1 2} Muhammad Naeem Ashiq³ Muhammad Azeem Ullah^{1 4} Shahid Iqbal⁵ Muhammad Latif⁵ Muhammad Ali⁶ Muhammad Fahad Ehsan⁷ Furhan Iqbal^5†

¹Institute of Advanced Materials, Bahauddin Zakariya University, Multan 60800, Pakistan ²College of Engineering, Mathematics and Physical Sciences, University of Exeter, EX4 4QF United Kingdom, Pakistan ³Institute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan ⁴, Pakistan ⁵Institute of Pure and Applied Biology, Zoology Division. Bahauddin Zakariya University, Multan 60800, Pakistan ⁶Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan 60800, Pakistan ⁷National Center for Nanoscience and Technology (NCNST), Beijing, China

furhan.iqbal@bzu.edu.pk

Korean Journal of Chemical Engineering, July 2016, 33(7), 2222-2227(6), 10.1007/s11814-016-0043-4

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Abstract

Present study was conducted to study the in vivo biocompatibility of cobalt iron oxide magnetic nano-particles (CoFe2O4 MNPs) in rabbits. CoFe2O4 MNPs were synthesized by the conventional micro emulsion technique in crystallite size range of 30 to 50 nm. The lattice constant (a) and cell volume were found to be 8.386 A and 589.75 A3, respectively, revealed by XRD. Subject animals were divided in three groups--low dose, high dose and control group without nanoparticles implantation for biocompatibility evaluation. CoFe2O4 was intraperitoneally implanted in rabbits: low dose (1mg CoFe2O4/Kg body weight) and high dose (10mg CoFe2O4/Kg body weight). Blood, serum and histological study of vital organs (liver, heart, kidney and spleen) were carried out in seven days of time protocol after sacrificing of animals. Results indicated that CoFe2O4 had drastically affected the blood chemistry in a dose-dependent manner as RDWa (P=0.01), Platelet (P<0.001) and Plateletcrit (P<0.001) concentrations reduced significantly in low dose and high dose CoFe2O4 treatments as compared to sham treated control group. Histological analysis revealed that CoFe2O4 exposure resulted in disordered and abnormal histology of liver, kidney and that of muscles at surgical site. It is concluded that CoFe2O4 has low biocompatibility and higher toxicity levels in living system at the applied doses.

Keywords

CoFe2O4 Micro Emulsion Synthesis Male Rabbit Hematology Serum Biochemistry Histology