Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received January 11, 2016
Accepted April 14, 2016

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Optimization of algal lipid extraction by mixture of ethyl acetate and ethanol via response surface methodology for biodiesel production

Weidong Lu^{1 2} Md Asraful Alam² Ying Pan^{2 3} William Junior Nock⁴ Zhongming Wang^{2 5†} Zhenhong Yuan^{2 5}

¹School of Chemistry and Environmental Engineering, Shaoguan University, Shaoguan 512005, China ²Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China ³Nano Science and Technology Institute, University of Science and Technology China, Suzhou 215123, China ⁴Faculty of Engineering and the Environment, University of Southampton, Southampton, SO17 1BJ, UK ⁵, China

wangzm@ms.giec.ac.cn

Korean Journal of Chemical Engineering, September 2016, 33(9), 2575-2581(7), 10.1007/s11814-016-0101-y

Download PDF

Abstract

The effects of extraction time, extraction temperature, solvent to biomass ratio and solvent composition on lipid yield from lyophilized Chlorococcum sp. biomass using a mixture of ethyl acetate and ethanol (EAE), a new proposed solvent, were studied. Subsequently, the process conditions of extraction by EAE were optimized using Box-Behnken design (BBD). The results revealed that the extraction temperature had the greatest effect on lipid extraction efficiency, followed by volume ratio of ethyl acetate to ethanol (EA/E) and extraction time. The largest lipid extraction yield of 15.74% was obtained under the following extraction conditions: 40mL solvents per gram of biomass for 270 min with gentle stirring at 80 ℃ by EAE with an EA/E of 1.0. Furthermore, palmitic acid, stearic acid, oleic acid, and linoleic acid were the most abundant fatty acids in the lipids extracted, indicating the great potential of the proposed lipid extraction procedure for microalgae-based biodiesel production.

Keywords

Biomass Lipids Microalgae Biodiesel

References

Demirbas A, Demirbas MF, Energy Conv. Manag., 52(1), 163 (2011)
Mofijur M, Masjuki HH, Kalam MA, Atabani AE, Shahabuddin M, Palash SM, Hazrat MA, Renew. Sust. Energ. Rev., 28, 441 (2013)
Haik Y, Selim MYE, Abdulrehman T, Energy, 36(3), 1827 (2011)
Suganya T, Varman M, Masjuki HH, Renganathan S, Renew. Sust. Energ. Rev., 55, 909 (2016)
Huo SH, Wang ZM, Zhu SN, Zhou WZ, Dong RJ, Yuan ZH, Bioresour. Technol., 121, 76 (2012)
Alam MA, Wan C, Zhao XQ, Chen LJ, Chang JS, Bai FW, J. Hazard. Mater., 289, 38 (2015)
Metting FB, J. Ind. Microbiol. Biotechnol., 17, 477 (1996)
Caporgno MP, Taleb A, Olkiewicz M, Font J, Pruvost J, Legrand J, Bengoa C, Algal Res., 10, 232 (2015)
Zhao BT, Su YX, Zhang YX, Cui GM, Energy, 89, 347 (2015)
Wijffels RH, Barbosa MJ, Science, 329(5993), 796 (2010)
Chisti Y, Trends Biotechnol., 26, 126 (2008)
Wan C, Alam MA, Zhao XQ, Zhang XY, Guo SL, Ho SH, Chang JS, Bai FW, Bioresour. Technol., 184, 251 (2015)
Halim R, Danquah MK, Webley PA, Biotechnol. Adv., 30, 709 (2012)
Halim R, Gladman B, Danquah MK, Webley PA, Bioresour. Technol., 102(1), 178 (2011)
Cheng CH, Du TB, Pi HC, Jang SM, Lin YH, Lee HT, Bioresour. Technol., 102(21), 10151 (2011)
Kanda H, Li P, Ikehara T, Yasumoto-Hirose M, Fuel, 95(1), 88 (2012)
Lu WD, Wang ZM, Yuan ZH, Bioresour. Technol., 191, 433 (2015)
Abubakar LU, Mutie AM, J. Appl. Phytotechnol. Environ. Sanit., 1, 147 (2012)
Indarti E, Majid MIA, Hashim R, Chong A, J. Food Compos. Anal., 18, 161 (2005)
Gan CY, Latiff AA, Food Chem., 124, 1277 (2011)
Tomsik A, Pavlic B, Vladic J, Ramic M, Brindza J, Vidovic S, Ultrason. Sonochem., 29, 502 (2016)
Ryckebosch E, Muylaert K, Foubert I, J. Am. Oil Chem. Soc., 89, 189 (2012)
Shi J, Yu J, Pohorly J, Young JC, Bryan M, Wu Y, J. Food Agric. Environ., 1(2), 42 (2003)
Meullemiestre A, Petitcolas E, Maache-Rezzoug Z, Chemat F, Rezzoug SA, Ultrason. Sonochem., 28, 230 (2016)
Quanhong L, Caili F, Food Chem., 92, 701 (2005)
Bligh EG, Dyer WJ, Can. J. Biochem. Physiol., 37, 911 (1959)