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Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received March 14, 2016
Accepted July 30, 2016

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kinetics of growth on dual substrates, production of novel glutaminase-free L-asparaginase and substrates utilization by Pectobacterium carotovorum MTCC 1428 in a batch bioreactor

Kumar Sanjay^{1 2} Ashish Prabhu Anand^{1 3} Venkata Dasu Veeranki^{1 3†} Pakshirajan Kannan^{1 3}

¹Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India ²Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad 211004 U.P., India ³, India

veeranki@iitg.ernet.in

Korean Journal of Chemical Engineering, January 2017, 34(1), 118-126(9), 10.1007/s11814-016-0216-1

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Abstract

Bacterial L-asparaginase has been widely used as a potential therapeutic agent in the treatment of various lymphoblastic leukemia diseases. We studied product and dual substrates utilization kinetics by P. carotovorum MTCC 1428 in batch bioreactor. The kinetic study revealed that the maximum growth of P. carotovorum MTCC 1428 was achieved at 2 g l-1 and 5 g l-1 of glucose and L-asparagine, respectively. Different substrate inhibition models were fitted to the growth kinetic data and the additive form of double Luong model was found to best explain the growth kinetics of P. carotovorum MTCC 1428. The kinetic parameters of growth studies showed that the predicted maximum inhibition concentration of glucose (Smg) and L-asparagine (Sma) was close to the experimentally observed value 15.0 and 10 gl-1, respectively. Modified form of the Luedeking-Piret model was used to describe the kinetics of L-asparaginase production, and the system seems to be mixed growth associated. Kinetic models of dual substrate growth, L-asparaginase production and substrate(s) utilization by P. carotovorum MTCC 1428 well fitted with experimental data with regression coefficients (R2) value of 0.97, 0.96 and 0.93, respectively.

Keywords

Anti-leukemic Enzyme L-asparaginase Pectobacterium carotovorum Growth Kinetics Multiple-substrate Production Kinetics

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