A new biocatalyst consisting of glucose oxidase (GOx) and polyethylenimine (PEI) immobilized on carbon nanotube (CNT) (CNT/PEI/GOx) was developed, while cyclic voltammogram (CV) behaviors of several related catalysts including the CNT/PEI/GOx were analyzed in terms of charge transfer resistances (Rcts) obtained by measuring Nyquist plots using electrochemical impedance spectroscopy (EIS). A qualitative correlation between the flavin adenine dinucleotide (FAD) redox reactivity measured by the CV and Rct was established. As factors affecting both the FAD reactivity and Rct, concentrations of GOx, glucose, and phosphate buffer solution, electrolyte pH and ambient condition were considered and evaluations of the catalysts using the CV curves and Nyquist plots confirmed that a pattern in the FAD reactivity was closely linked to that in the Rct, implying that FAD reactivities of the catalysts are predicted by the measurements of their Rcts. Even regarding performance of the enzymatic biofeul cells(EBCs) using the reacted catalysts, a pattern of the Rcts is compatible with that in the maximum power densities (MPDs) of the EBCs.

Glucose Oxidase Charge Transfer Resistance Flavin Adenine Dinucleotide Redox Reactivity Enzymatic Biofuel Cell Cyclic Voltammogram

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