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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received June 29, 2016
Accepted November 25, 2016

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Simultaneous saccharification and fermentation of alkali-pretreated corncob under optimized conditions using cold-tolerant indigenous holocellulase

Anamika Sharma^{1 2} Vikrant Nain² Rameshwar Tiwari^{1 3} Surender Singh^{1 3} Anurup Adak^{1 3} Pawan Kumar Singh Nain⁴ Lata Nain^{1 3†}

¹Division of Microbiology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India ²School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh-201312 India ³, India ⁴Design and Mechatronic Division, School of Civil and Mechanical Engineering, Galgotias University, Uttar Pradesh-201312, India

Korean Journal of Chemical Engineering, March 2017, 34(3), 773-780(8), 10.1007/s11814-016-0334-9

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Abstract

The present investigation was aimed towards pretreatment optimization of corncob to maximize cellulose and hemicellulose recovery, followed by substrate selection for holocellulase production using psychrotolerant Aspergillus niger SH3. Dilute alkali pretreatment (1.5% NaOH) resulted in higher recovery of cellulose (59.66%) and hemicellulose (28.34%) from corncob, while corn stover proved to be the best substrate for holocellulase production. Further, saccharification was optimized by Box-Behnken design to select the suitable conditions for maximum sugar release from pretreated corncob. The optimum conditions for maximum sugar release were 8% (w/v) substrate loading, 11 FPU/gds enzyme loading at temperature 38 °C and pH 3.0 which resulted in 114.5% higher sugar yield (912mg/gds of pretreated biomass) as compared with un-optimized conditions (425.35mg/gds). Theoretical yield of 48.8% ethanol was achieved through simultaneous saccharification and fermentation (SSF) using pretreated corncob. This study illustrates the potential of different corn residues as a promising substrate for bioethanol production.

Keywords

Bioethanol Corn residues Aspergillus niger Saccharification Fermentation Box-Behnken

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