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Received October 24, 2016
Accepted November 20, 2016
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Comparison of metabolite profiling of Ralstonia eutropha H16 phaBCA mutants grown on different carbon sources
1Department of Chemical and Biological Engineering, Korea University, Seoul 02841, Korea 2CJ Research Institute of Biotechnology, Suwon 16495, Korea 3Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 04107, Korea 4, Korea
jinwonlee@sogang.ac.kr
Korean Journal of Chemical Engineering, March 2017, 34(3), 797-805(9), 10.1007/s11814-016-0333-x
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Abstract
Metabolite profiling was conducted to monitor the metabolic differences of the Ralstonia eutropha H16, polyhydroxybutyrate (PHB) producing strain, when grown on different sole carbon sources and to identify the effect of the deletion of the PHB production pathway. The combination of rapid filtration and boiling ethanol extraction successfully extracted intracellular metabolites. Accumulation of acetyl-CoA was detected when the strain was grown on fructose. Whereas, lower adenylate energy charge (AEC) ratio and cell mass was identified on the cells grown on acetate. No significant difference was detected between the wild type and mutant strain except metabolites in the PHB synthetic pathway. Increased PHB production is expected on fructose due to the accumulation of AcCoA, while it is more difficult to improve the production on acetate because of lower metabolic precursor level and AEC ratio.
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References
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