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Received November 27, 2016
Accepted March 5, 2017
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Construction of methanol sensing Escherichia coli by the introduction of novel chimeric MxcQZ/OmpR two-component system from Methylobacterium organophilum XX
Vidhya Selvamani1
Murali Kannan Maruthamuthu1
Kulandaisamy Arulsamy2
Gyeong Tae Eom3 4
Soon Ho Hong1†
1Department of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Namgu, Ulsan 44610, Korea 2Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India 3Research Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), 45 Jongga-ro, Jung-gu, Ulsan 44429, Korea 4Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34144, Korea
hhong@ulsan.ac.kr
Korean Journal of Chemical Engineering, June 2017, 34(6), 1734-1739(6), 10.1007/s11814-017-0063-8
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Abstract
Methylobacterium organophilum XX is a type II facultative methylotroph that can grow on methanol. In M. organophilum XX, the MxcQ/MxcE two-component system (TCS) is involved in methanol metabolism. EnvZ/OmpR in E. coli TCS was exploited to develop a methanol biosensor by engaging the MxcQ/MxcE TCS system. The MxcQZ/ OmpR methanol sensing chimeric TCS was constructed by integrating the sensing domain of M. organophilum MxcQ with the transmitter domain of E. coli EnvZ. The response regulator of the chimeric TCS system is OmpR, which regulates the expression of the ompC and gfp. The expression of ompC was monitored by real-time quantitative PCR analysis. The expression of gfp also confirmed the expression of the ompC. The maximum expression of ompC and gfp occurred with 0.05% of methanol, and the expression started to decline with further increases in methanol concentration. This system delivers rapid detection of methanol in the environment.
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