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Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received October 19, 2017
Accepted November 20, 2017

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Strategies for the optimization of bead-immunoassays for the effective detection of target biomolecules

Jaewoo Son Jangsun Hwang Dohyun Lee Muhammad Saad Khan Yeonho Jo Kyungwoo Lee Chanhwi Park Sachin Chavan Youngmin Seo¹ Younghyun Choi Sangsoo Kim Dasom Kim Dokyun Na^† Jonghoon Choi^†

School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea ¹Center for Biomaterials, Boimedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, Korea

nanomed@cau.ac.kr

Korean Journal of Chemical Engineering, March 2018, 35(3), 805-811(7), 10.1007/s11814-017-0323-7

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Abstract

Immunoassays are analytical methods using antibody-specific reactions to analyze samples. Due to recent developments in antibody technology, the scope of potential samples has expanded to not only proteins, but also low molecular-weight compounds, carbohydrates, lipids, and microorganisms. Immunoassays have the advantage of being highly sensitive, capable of detecting small amounts, and thus have potential for application in biosensors. Immunoassays using magnetic beads have been developed and can be converted to more diverse platforms than the existing limited well plate-based assay. Furthermore, magnetic bead immunoassays detect analytical samples more quickly, and are becoming one of the most suitable immunoassay tools applicable to biosensors. However, their development requires optimization for the improvement of detection ability for specific samples. Therefore, we propose a guideline for solving detection problems occurring in magnetic bead immunoassay optimization processes. It is aimed to be a good reference, enabling researchers performing such optimization more quickly and efficiently.

Keywords

Immunoassay Magnetic Bead Assay Optimization Colorimetric Assay Biosensor

References

Darwish IA, Int. J. Biomed. Sci., 2, 217 (2006)
Bonwick GA, Smith CJ, Int. J. Food Sci. Technol., 39, 817 (2004)
Lequin RM, Clin. Chem., 51, 2415 (2005)
Wu AH, Clin. Chim. Acta, 369, 119 (2006)
Lien KY, Hung LY, Huang TB, Tsai YC, Lei HY, Lee GB, Biosens. Bioelectron., 26, 3900 (2011)
Rønning OW, Christophersen AC, Hybridoma, 10, 641 (1991)
Aytur T, Foley J, Anwar M, Boser B, Harris E, Beatty PR, J. Immunol. Methods, 314, 21 (2006)
Jackson T, Ekins R, J. Immunol. Methods, 87, 13 (1986)
Nikitin P, Vetoshko P, Ksenevich T, Sens. Lett., 5, 296 (2007)
Gundersen S, Haagensen I, Jonassen T, Figenschau K, De Jonge N, Deelder A, J. Immunol. Methods, 148, 1 (1992)
Zhao F, Shen Q, Wang H, Han X, Yang Z, Food Control, 79, 227 (2017)
Radoi A, Targa M, Prieto-Simon B, Marty JL, Talanta, 77, 138 (2008)
Yu S, Yu F, Li Y, Liu L, Zhang H, Qu L, Wu Y, Food Control, 60, 500 (2016)
Jung DH, Min K, Jeon Y, Jang W, Kwon Y, Biochip J., 6, 293 (2012)
Andreassen J, Immunodiagnostic, 29, 22 (2005)
Perraut R, Varela M, Mbengue B, Guillotte M, Mercereau-Puijalon O, Vigan-womas I, J. Immunol. Tech. Infect. Dis., 4, 1 (2015)