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Received May 7, 2017
Accepted December 30, 2017
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Enhanced D-ribose production by genetic modification and medium optimization in Bacillus subtilis 168
Key Laboratory of Food and Fermentation Engineering of Shandong Province, Shandong Food Ferment Industry Research & Design Institute, Jinan 250013, P. R. China
Korean Journal of Chemical Engineering, May 2018, 35(5), 1137-1143(7), 10.1007/s11814-017-0356-y
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Abstract
D-ribose, a five-carbon sugar with diverse applications, is mainly produced by transketolase(tkt)-deficient Bacillus subtilis (B. Subtilis). We constructed B.subtilis SFR-3A by replacing the corresponding sites of B. subtilis 168 with the mutation site of tkt in the “wild” D-ribose high-producing strain B. subtilis SFR-4, resulting in 5.29 g/L of Dribose. In the meantime, B.subtilis SFR-159 was constructed by completely removing the tkt gene of B. subtilis 168 with a higher production of 6.21 g/L. Through medium optimization, batch fermentation of SFR-3A and SFR-159 gave the best result of 27.56 g/L and 29.89 g/L, which corresponds to productivity of 0.51 g/L/h and 0.41 g/L/h, respectively. In this work, SFR-3A showed more latent capacity over SFR-159 as to productivity and had greater potential to serve as a platform for D-ribose production.
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