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Received December 11, 2018
Accepted April 9, 2019
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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The mutation of Scenedesmus obliquus grown in municipal wastewater by laser combined with ultraviolet
Xu Zhou
Wenbiao Jin†
Song-fang Han
Xiaoye Li
Shu-Hong Gao1
Chuan Chen2
Guo-jun Xie2
Renjie Tu
Qing Wang
Qilin Wang3
Shenzhen Engineering Laboratory of Microalgal Bioenergy, Harbin Institute of Technology (Shenzhen), 518055 Shenzhen, China 1Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma 73019, USA 2State Key Laboratory of Urban Water Resource and Environment (SKLUWRE), Harbin Institute of Technology, 150001, Harbin, China 3Centre for Technology in Water and Wastewater, School of Civil and Environmental Engineering, University of Technology Sydney, Ultimo, NSW 2007, Australia
jinwb@hit.edu.cn
Korean Journal of Chemical Engineering, June 2019, 36(6), 880-885(6), 10.1007/s11814-019-0273-3
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Abstract
Mutagenetic breeding is an efficient technique for the enhancement of lipid productivity from microalgae. In this study, oil-rich microalga Scenedesmus obliquus were treated by Laser-UV composite mutagenesis. Among the 35 mutant strains, X5 was primely screened. Afterwards, a twice UV mutagenizing was operated on X5, and the optimal mutant strain X5-H13 was obtained. The growth rate, dry weight, lipid yield and lipid content of X5-H13 were 0.698× 107 cells/mL·d, 0.99 g/L, 0.49 g/L and 48.8% while cultivated in municipal wastewater, respectively, which were increased by 45%, 58%, 109% and 32% than the original strain. The results of the subculture of repeated mutant showed that the biomass and lipid content of strain X5-H13 were up to 0.99 g/L and 48.8%. The growth of each generation was stable. Furthermore, the random amplified polymorphic DNA analysis indicated that the mutant strain X5-H13 was different from the starting strain, with their genetic similarity coefficient value of 0.815.
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