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Received December 31, 2019
Accepted April 30, 2020
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Simultaneous accumulation of lipid and carotenoid in freshwater green microalgae Desmodesmus subspicatus LC172266 by nutrient replete strategy under mixotrophic condition
1Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria 2Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
Korean Journal of Chemical Engineering, September 2020, 37(9), 1522-1529(8), 10.1007/s11814-020-0564-8
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Abstract
Production of biofuel by microalgae can become economically viable if other high-value commercial products such as carotenoid are simultaneously produced. This study aimed at enhancement of lipid and carotenoid productivity by an oleaginous microalga sp. Desmodesmus subspicatus LC172266 by supplementing Nitrogen sources to BG 11 medium under batch or fed batch mixotrophic condition. Optimum urea supplement (0.2 gL-1) to BG 11 medium enhanced carotenoid content of the D. subspicatus by about 3.7 fold (3.02±0.002mgg-1 cell) and 3.0 fold (3.86±0.001mgg-1 cell) using glucose or glycerol as organic carbon sources, respectively. Also, lipid productivity was enhanced by about 2.0 (0.07817±0.0028 gL-1day-1) (0.265±0.006 gg-1 cell) and 2.5 (0.08426±0.0008 gL-1day-1) (0.285± 0.02 gg-1 cell) fold. Fed-batch cultivation with glucose at a feed rate of 0.5 gL-1 per day enhanced both carotenoid and lipid productivities of D. subspicatus by 34% and 45%, respectively, after eight days of mixotrophic cultivation.
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References
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Koyande AK, Show PL, Guo R, Tang B, Ogino C, Chang JS, Bioenerg, 10, 574 (2019)
Pugazhendhi A, Shobana S, Bakonyi P, Nemestothy N, Xia A, Banu RJ, Kumar G, Biotechnol. Rep., 20, e00302 (2018)
Wijanarko A, in Progress in biomass and bioenergy production, InTech, London (2011).
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Cordero BF, Obraztsova I, Couso I, Mar. Drugs., 9, 1607 (2011)
Kumar BR, Deviram G, Mathimani T, Duc PA, Pugazhendhi A, Biocat. Agri. Biotechnol., 17, 583 (2019)
Eze CN, Ogbonna JC, Ogbonna IO, Aoyagi H, J. Appl. Phycol., 29, 2745 (2017)
Chen CY, Ho SH, Liu CC, Chang JS, J. Taiwan Inst. Chem. Eng., 79, 88 (2017)
Kumar DA, Gopul T, Harinath K, Sibi G, SOJ Microbiol. Infect. Dis., 5, 1 (2017)
Cezare-Gomes EA, Meji-da-Silva LDC, Perez-Mora LS, et al., Appl. Biochem. Biotechnol., 188, 602 (2018)
Bligh EG, Dyer WJ, J. Biochem. Physiol., 37, 911 (1959)
Caprio FD, Visca A, Altimari P, Toro L, Barbara M, Laquaniello G, Pagnanelli F, Chem. Eng. Trans., 49, 415 (2016)
Minyuk G, Sidorov R, Solovchenko A, J. Appl. Phycol., 32, 935 (2020)
Minyuk GS, Dantsyuk NV, Chelebieva ES, Chubchikova IN, Drobetskaya IV, Solovchenko AE, Mar. Biol. J., 4, 4 (2019)
Lichtenthaler HK, Methods Enzymol., 148, 350 (1987)
Yen HW, Chang JT, Bioproc. Biosyst. Eng., 36, 1797 (2013)
Abdulsamad JK, Varghese SA, Thajudeen J, J. Microbiol. Biotechnol. Food Sci., 9, 3 (2019)
Benavente-Valdes JR, Aguilar C, Contrares-Esquivel JC, Memvadez-Zavala A, Montanez J, Biotechnol. Rep., 10, 117 (2016)
Sharma J, Kumar SS, Bishnoi NR, Pugazhendhi A, J. Mol. Liq., 269, 712 (2018)
Sharma J, Kumar V, Kumar SS, Malyan SK, Mathimani T, Bishnoi NR, Pugazhendhi A, J. Phytochem. Photobiol., 202, 111638 (2020)
Guyon JB, Verge V, Schatt P, Lozano JC, Liennard M, Bouget FY, Mar. Drugs, 16, 79 (2018)
Chen JH, Chen CY, Hasunuma T, Kondo A, Chang CH, Ng IS, Chang JS, Bioresour. Technol., 278, 17 (2019)
Chen L, Zhang LL, Liu TZ, Bioresour. Technol., 214, 567 (2016)
Novoveska L, Ross ME, Stanley MS, Pradelles R, Wasiolek V, Sassi JF, Mar. Drugs., 17, 640 (2019)
Ramanna L, Guldhe A, Rawat I, Bux F, Bioresour. Technol., 98, 560 (2014)
Zhu L, Li S, Hu T, Nugroho YK, Yin Z, Hu D, Chu R, Mo F, Liu C, Hilunen E, Energy Conv. Manag., 201, 112144 (2019)
Zarrinmehr MJ, Farhadian O, Heyrati FP, Keramat J, Koutra E, Kornaros M, Daneshvar E, Egyp. J. Aquatic Res., 11, 1687 (2019)
Ismaiel MMS, El-Ayouty YM, Said AA, Fathey HA, Biocat. Agri. Biotechnol., 16, 378 (2018)
Song MM, Pei HY, Bioresour. Technol., 263, 525 (2018)
Bongiovani N, Popovich CA, Martinez AM, Constenla D, Leonardi PI, Bioenerg. Res., 13, 529 (2020)
Hossain N, Mahila TMI, Saidur R, Biotechnol. Biofuel., 12, 125 (2019)
Chi NTL, Duc PA, Mathimani T, Pugazhendhi A, Biocat. Agri. Biotechnol., 17, 184 (2019)
Sathasivam R, Ki JS, Mar. Drugs., 16, 26 (2018)
Anto S, Mukharjee SS, Muthappa R, Mathimani T, Deviram G, Kumar SS, Verma TN, Pugazhendhi A, Chemosphere, 242, 125079 (2019)
Chakravarty S, Mallick N, Biomass Bioeng., 126, 1 (2019)
Dhup S, Kannan DC, Dhawan V, SOJ Biochem., 2, 1 (2016)
Mandal S, Shurin JB, Efroymson RA, Mathews TJ, Ecosphere, 9, e02228 (2018)
Braunwald T, Schwemmlein L, Graeff-Honninger S, French WT, Hernandez R, Holmes WE, Claupein W, Appl. Micribiol. Biotechnol., 97, 6581 (2013)
Li TT, Zheng YB, Yu L, Chen SL, Bioresour. Technol., 131, 60 (2013)
Mathimani T, Baldineli A, Rajendran K, Prabakar D, Matheswaran M, van Leeuwen RP, Pugazhendhi A, J. Clean Prod., 208, 1053 (2019)
Mathimani T, Pugazhendhi A, Biocat. Agri. Biotechnol., 17, 326 (2019)
Mathimani T, Umar L, Prabaharan D, J. Clean Prod., 198, 575 (2018)
Shi XM, Zhang XW, Chen F, Enzyme Microb. Technol., 27(3-5), 312 (2000)
Zhao XF, Jarboe L, Wen ZY, Appl. Microbiol. Biotechnol., 100(9), 4241 (2016)
Ye YL, Huang Y, Xia A, Fu Q, Liao Q, Zeng WD, Zheng YP, Zhu X, Bioresour. Technol., 270, 80 (2018)
Xie Y, Li J, Ma R, Ho SH, Shi X, Liu L, Chen J, Bioresour. Technol., 290, 121798 (2019)
Xie YP, Ho SH, Chen CNN, Chen CY, Ng IS, Jing KJ, Chang JS, Lu YH, Bioresour. Technol., 144, 435 (2013)
