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Received June 17, 2020
Accepted September 18, 2020
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Sugaring-out extraction of erythromycin from fermentation broth
Department of Chemical Engineering, Visvesvaraya National Institute of Technology, Nagpur, Maharashtra India
pdhamole@che.vnit.ac.in, pradipdhamole@gmail.com
Korean Journal of Chemical Engineering, January 2021, 38(1), 90-97(8), 10.1007/s11814-020-0680-5
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Abstract
This study reports the sugaring-out extraction of erythromycin from fermentation broth using acetonitrile (ACN) as solvent and glucose as a mass separating agent. Different process parameters--glucose concentration, temperature, ACN/water ratio and pH--were optimized to achieve maximum extraction of erythromycin. 88% (w/w) of erythromycin was extracted from the model system with following optimized conditions: glucose 156.3 g/L; temperature 4 °C; ACN/water ratio 1 and pH 8.3. Further, the effect of typical fermentation media components (starch, soybean flour, CaCO3, NaCl and (NH4)2SO4) on sugaring out extraction of erythromycin was also investigated. Starch, soybean flour and CaCO3 were observed to affect erythromycin extraction only at higher concentration. Removal of suspended solids from simulated as well as real broth prior to extraction enhanced the extraction efficiency (from 72% to 87%). Sugaring out extraction of erythromycin was found to be more effective than salting out extraction. Also, higher partition coefficient was achieved in the present work than other reported methods using carbohydrates as mass separating agent. Further, it was found that the antimicrobial activity of erythromycin was preserved during sugaring out extraction of erythromycin.
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Sanghvi GV, Ghevariya D, Gosai S, Langa R, Dhaduk N, et al., Biotechnol. Reports, 1-2, 2 (2014)
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Nabais AMA, Cardoso JP, Bioprocess Eng., 21, 157 (1999)
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Lightfoot EN, Moscariello JS, Bioseparations, Biotechnol. Bioeng., 87, 259 (2004).
Jones LA, Prabel JB, Glennon JJ, Copeland MF, Kavlock RJ, J. Agric. Food Chem., 41, 735 (1993)
Leinonen H, Corrosion, 52, 337 (1996)
Gu YS, Shih PH, Enzyme Microb. Technol., 35(6-7), 592 (2004)
Wang B, Ezejias T, Feng H, Blaschek H, Chem. Eng. Sci., 63(9), 2595 (2008)
Wang B, Feng H, Ezeji T, Blaschek H, Chem. Eng. Technol., 31(12), 1869 (2008)
Dhamole PB, Mahajan P, Feng H, J. Chem. Eng. Data, 55(9), 3803 (2010)
Dai JY, Liu CJ, Xiu ZL, Process Biochem., 50(11), 1951 (2015)
Yan L, Sun YQ, Xiu ZL, Sep. Purif. Technol., 161, 152 (2016)
Dai JY, Ma LH, Wang ZF, Guan WT, Xiu ZL, Bioprocess. Biosyst. Eng., 40, 423 (2017)
Sun YQ, Zhang SS, Zhang XX, Zheng YF, Xiu ZL, Sep. Purif. Technol., 204, 133 (2018)
Sun YQ, Zhang XX, Zheng YF, Yan L, Xiu ZL, Sep. Purif. Technol., 209, 972 (2019)
Shoushtari BA, Pazuki G, Shahrouzi JR, Shahriari S, Hadidi N, Fluid Phase Equilib., 505, 112360 (2020)
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Cardoso GDB, Souz IN, Pereira MM, Freire MG, Soares CMF, Lima AS, Sep. Purif. Technol., 136, 74 (2014)
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Balouiri M, Sadiki M, Ibnsouda SK, J. Pharm. Anal., 6, 71 (2016)
Anuar N, Adnan AFM, Saat N, Aziz N, Taha RM, Scientific World J., 2013, 810547 (2013)
Koley S, Ghosh S, ChemPhysChem., 16, 3518 (2015)
Manic MS, da Ponte MN, Najdanovic-Visak V, Chem. Eng. J., 171(3), 904 (2011)
