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Received July 8, 2020
Accepted September 1, 2020
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Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel
Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Korea 1Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Korea
biochoi@cnu.ac.kr
Korean Journal of Chemical Engineering, January 2021, 38(1), 98-103(6), 10.1007/s11814-020-0672-5
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Abstract
Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an engineered tyrosinase (mTyr-CNK) with high catalytic activity for tyrosine/ DOPA-tethered polymeric biomaterials throughout a broad pH range. A dual-barrel syringe with one part containing chitosan/mTyr-CNK solution and the other containing gelatin solution with/without nanohydroxyapatite was successfully used to form homogeneous hydrogels at room temperature followed by 37 °C to simulate an in situ injection approach. The obtained hydrogels exhibited an average pore size greater than 150 μm and high swelling ratios with similar mechanical properties to other chemically crosslinked chitosan/gelatin hydrogels. The in vitro degradation properties and cellular viability suggested that the hydrogels could be used as biodegradable and biocompatible scaffolds for biomedical applications, such as space filling biomaterials and delivery vehicles for bioactive molecules and cells. These results demonstrated that mTyr-CNK-mediated hydrogels have remarkable promise as an injectable scaffold biomaterial.
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References
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Hafidz RNRM, Yaakob CM, Amin I, Noorfaizan A, Int. Food Res. J., 18, 787 (2011)
Kim EH, Lim S, Kim TE, Jeon IO, Choi YS, Biotechnol. Bioprocess Eng., 23, 500 (2018)
Yan CQ, Pochan DJ, Chem. Soc. Rev., 39, 3528 (2010)
Guo L, Colby RH, Lusignan CP, Howe AM, Macromolecules, 36(26), 10009 (2003)
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