Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received March 11, 2021
Accepted July 30, 2021
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production
School of Engineering, RMIT University, Melbourne VIC 3001, Australia 1Department of Mechanical Engineering, Universiti Tenaga Nasional, Selangor 43000, Malaysia 2Department of Electronic Materials Engineering, Kwangwoon University, Seoul 01897, Korea 3Department of Mechanical Engineering, School of Technology, Glocal University, Uttar Pradesh 247121, India 4School of Information, Systems and Modelling, Faculty of Engineering and Information Technology, University of Technology Sydney, NSW 2007, Australia
Korean Journal of Chemical Engineering, December 2021, 38(12), 2493-2499(7), 10.1007/s11814-021-0914-1
Download PDF
Abstract
Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H2SO4) and hydrolyzed with one of the most available and cost-effective enzymes, cellulase derived from Trichoderma reesei, under submerged static condition. Three different pretreatment approaches--cut into 2 cm2, blended with distilled water, and pretreated with diluted H2SO4--have been implemented, and pretreatment with diluted H2SO4 was the most effective. Hydrolysis with different concentrations--0.5M, 1.0M, 1.5M, 2.0M of H2SO4--was performed. The maximum glucose content was obtained at 2.0M H2SO4 at 90 min reaction time, and glucose yield was 0.11 g glucose/ g wastepaper. The cut paper, wet-blended, and acid-treated wastepaper was hydrolyzed with cellulase enzyme for 2, 4, and 5 consecutive days with 5mg, 10mg, 15mg, and 20mg enzyme loadings. The maximum glucose content obtained was 9.75 g/l from acid-treated wastepaper, after 5 days of enzymatic hydrolysis with 20mg enzyme loading and a glucose yield of a 0.5 g glucose/g wastepaper. The wastepaper hydrolysate was further fermented for 6, 8, and 10 hours continuously with Saccharomyces cerevisiae (yeast), and at 10 hours of fermentation, the maximum glucose consumption was 0.18 g by yeast. Further, HPLC analysis of the fermented medium presented a strong peak of bioethanol content at 16.12min. The distillation of bioethanol by rotary evaporator presented 0.79ml bioethanol/fermented solution, which indicated the conversion efficiency of 79%.
References
Lim S, Teong LK, Renew. Sust. Energ. Rev., 14, 938 (2010)
Balasbaneh AT, Marsono AKB, Khaleghi SJ, J. Building Eng., 20, 235 (2018)
Hossain N, Razali AN, Mahlia TMI, Chowdhury T, Chowdhury H, Ong HC, Shamsuddin AH, Silitonga AS, Energies, 12, 3947 (2019)
Ahorsu R, Medina F, Constanti M, Energies, 11, 3366 (2018)
Clauser NM, Gonzalez G, Mendieta CM, Kruyeniski J, Area MC, Vallejos ME, Sustainability, 13, 794 (2021)
Muller J, Waste disposal rate in malaysia in 2020. https://www.statista.com/statistics/1133319/malaysia-waste-disposalrate-by-method/
Annamalai N, et al., Waste and Biomass Valorization, 11, 121 (2020).
Tadmourt W, Khiari K, Boulal A, Tarabet L, Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 1 (2020).
Byadgi SA, Kalburgi P, Procedia Environ. Sci., 35, 555 (2016)
Wang L, Sharifzadeh M, Templer R, Murphy RJ, Appl. Energy, 111, 1172 (2013)
Garg R, Srivastava R, Brahma V, Verma L, Karthikeyan S, Sahni G, Sci. Rep., 6, 1 (2016)
Hossain N, Zaini JH, Mahlia T, Int. J. Technol., 8, 5 (2017)
Lin CY, Peng MT, Tsai YC, Tsai SJ, Wu TY, Chien SY, Tsai HJ, Asian J. Agric. Food Sci., 3, 333 (2015)
Kusmiyati, Mustofa A, Jumarmi, IOP Conf. Series: Mater. Sci. Eng., 358 (2018).
Rocha J, Alencar B, Mota H, Gouveia E, Cell Chem. Technol., 50, 243 (2016)
Dubey AK, Gupta P, Garg N, Naithani S, Carbohydr. Polym., 88, 825 (2012)
Chua K, Sahid EJM, Leong Y, ST-4: Green & Energy Management, 4, 1 (2011).
Ioelovich M, J. Sci. Res. Rep., 905 (2014).
Balasbaneh AT, Marsono AKB, Khaleghi SJ, J. Building Eng., 20, 235 (2018)
Hossain N, Razali AN, Mahlia TMI, Chowdhury T, Chowdhury H, Ong HC, Shamsuddin AH, Silitonga AS, Energies, 12, 3947 (2019)
Ahorsu R, Medina F, Constanti M, Energies, 11, 3366 (2018)
Clauser NM, Gonzalez G, Mendieta CM, Kruyeniski J, Area MC, Vallejos ME, Sustainability, 13, 794 (2021)
Muller J, Waste disposal rate in malaysia in 2020. https://www.statista.com/statistics/1133319/malaysia-waste-disposalrate-by-method/
Annamalai N, et al., Waste and Biomass Valorization, 11, 121 (2020).
Tadmourt W, Khiari K, Boulal A, Tarabet L, Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 1 (2020).
Byadgi SA, Kalburgi P, Procedia Environ. Sci., 35, 555 (2016)
Wang L, Sharifzadeh M, Templer R, Murphy RJ, Appl. Energy, 111, 1172 (2013)
Garg R, Srivastava R, Brahma V, Verma L, Karthikeyan S, Sahni G, Sci. Rep., 6, 1 (2016)
Hossain N, Zaini JH, Mahlia T, Int. J. Technol., 8, 5 (2017)
Lin CY, Peng MT, Tsai YC, Tsai SJ, Wu TY, Chien SY, Tsai HJ, Asian J. Agric. Food Sci., 3, 333 (2015)
Kusmiyati, Mustofa A, Jumarmi, IOP Conf. Series: Mater. Sci. Eng., 358 (2018).
Rocha J, Alencar B, Mota H, Gouveia E, Cell Chem. Technol., 50, 243 (2016)
Dubey AK, Gupta P, Garg N, Naithani S, Carbohydr. Polym., 88, 825 (2012)
Chua K, Sahid EJM, Leong Y, ST-4: Green & Energy Management, 4, 1 (2011).
Ioelovich M, J. Sci. Res. Rep., 905 (2014).
