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Received January 16, 2021
Accepted February 26, 2021
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Regenerated coenzyme-based preparation of bienzyme-polymer nanoconjugates and their applications for the synthesis of ethyl (R)-2-hydroxy-4-phenylbutyrate
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang, China 1The Third Affiliated Hospital of Qiqihar Medical College, Qiqihar, Heilongjiang, China
708900599@qq.com
Korean Journal of Chemical Engineering, May 2021, 38(5), 1066-1077(12), 10.1007/s11814-021-0775-7
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Abstract
A modular approach was applied for the synthesis of bienzyme-polymer nanoconjugates (nano-BECs) (50-70 nm) consisting of two enzymes (carbonyl reductase and glucose dehydrogenase) conjugated within a single universal polymer scaffold. The amount of the product ethyl (R)-2-hydroxy-4-phenylbutyrate (R-HPBE) with nano-BECs as the catalyst was 533mM in a dibutyl phthalate-phosphate buffer (dibutyl phthalate-PB) biphasic system, while the amount of R-HPBE was 349mM using carbonyl reductase-poly(acrylic acid) as the catalyst, indicating that the nano- BECs have an advantage for coenzyme regeneration. Compared with a single aqueous phase, the substrate treatment capacity was improved at the interface of the dibutyl phthalate-PB biphasic system. Under the optimal reaction conditions (35 °C, 40 h, dibutyl phthalate-PB 1 : 1), nano-BECs can completely convert substrate into optically pure R-HPBE (enantiomeric excess (e.e.) >99.9%) in the organic-aqueous system.
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References
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