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Received December 8, 2020
Accepted March 12, 2021
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Biosynthesis of polyhydroxyalkanoates from sugarcane molasses by recombinant Ralstonia eutropha strains
Seo Young Jo
Yu Jung Sohn
Se Young Park
Jina Son
Jee In Yoo
Kei-Anne Baritugo
Yokimiko David
Kyoung Hee Kang1
Hoyong Kim2
Jong-il Choi3
Mi Na Rhie
Hee Taek Kim4†
Jeong Chan Joo5†
Si Jae Park†
Department of Chemical Engineering and Materials Science, Graduate Program in System Health Science and Engineering, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul 03760, Korea 1Bio-based Chemistry Center, Advanced Convergent Chemistry Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea 2Bio-based Chemistry Research Center, Advanced Convergent Chemistry Division, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44412, Korea 3Department of Biotechnology and Engineering, Interdisciplinary Program of Bioenergy and Biomaterials, Chonnam National University, Gwangju 61186, Korea 4Department of Food Science and Technology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea 5Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do 14662, Korea
heetaek@cnu.ac.kr
Korean Journal of Chemical Engineering, July 2021, 38(7), 1452-1459(8), 10.1007/s11814-021-0783-7
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Abstract
Sugarcane molasses was examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] in recombinant Ralstonia eutropha strains expressing Mannheimia succiniciproducens sacC gene encoding β-fructofuranosidase, which can hydrolyze sucrose into glucose and fructose in the culture medium. When crude sugarcane molasses was added to the culture medium to support 20 g/L of sucrose in flask cultivation, the growth of R. eutropha NCIMB11599 expressing the sacC gene was significantly inhibited, which resulted in OD600 of 1.2 with P(3HB) content of 0.1wt%. The inhibition of cell growth due to the usage of the crude sugarcane molasses was relieved by pretreatment of sugarcane molasses with activated charcoal. Sugarcane molasses pretreated with activated charcoal could support the growth of R. eutropha NCIMB11599 expressing the sacC gene to OD600 of 87.2 with P(3HB) content of 82.5 wt% in batch fermentation when it was added to culture medium to support 20 g/L of sucrose. Also, R. eutropha 437-540 expressing Escherichia coli ldhA gene encoding lactate dehydrogenase along with the sacC gene produced P(3HB-co-6.2mol%LA) with 29.1 wt% polymer content from sugarcane molasses in batch fermentation.
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Brauegg G, Sonnleitner B, Lafferty R, Eur. J. Appl. Microbiol. Biotechnol., 6(1), 29 (1978)
Gong C, Chen C, Chen L, Appl. Biochem. Biotechnol., 39(1), 83 (1993)
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Gupta R, Gautam S, Shukla R, Kuhad RC, J. Environ. Chem. Eng., 5(5), 4573 (2017)
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Sirisatesuwon C, Ninchan B, Sriroth K, Sugar Tech, 22(2), 274 (2020)
Ehrmann MA, Korakli M, Vogel RF, Curr. Microbiol., 46, 0391 (2003)
Tosun A, Ergun M, J. Chem. Technol. Biotechnol., 82(1), 11 (2007)
Chotineeranat S, Wansuksri R, Piyachomkwan K, Chatakanonda P, Weerathaworn P, Sriroth K, Sugar Tech, 12(2), 120 (2010)
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Baei MS, Najafpour G, Younesi H, Tabandeh F, Eisazadeh H, World Appl. Sci. J., 7(2), 157 (2009)
Ul-Islam M, Ullah MW, Khan S, Park JK, Korean J. Chem. Eng., 37(6), 925 (2020)
