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Received January 5, 2021
Accepted April 4, 2021
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Optimization study for enhanced production of hyaluronic acid from Streptococcus equisimilus MK156140
Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi Campus, Bangalore-560 056, India 1M.S. Ramaiah Institute of Technology, Bangalore-560054, India
Korean Journal of Chemical Engineering, September 2021, 38(9), 1880-1887(8), 10.1007/s11814-021-0798-0
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Abstract
Hyaluronic acid finds its complete application in areas such as therapeutics, cosmetics, and as a health supplement. In the present investigation, standardization for the production of hyaluronic acid by Streptococcus equisimilus MK156140 in complex media was performed. Some of the selected physicochemical parameters such as pH, temperature, speed, incubation time, sucrose, yeast extract, and beef extract were screened using Plackett-Burman foldover design. Further, the screened parameters interaction was investigated using central composite design (CCD) and closely compared with OVAT studies. At a pH of 7.38, with beef extract, 12.15%, and yeast extract 7.64%, the observed yield was 7.16 g/L, which was in close line with the predicted value of 7.21 g/L.
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Blank LM, McLaughlin RL, Nielsen LK, Biotechnol. Bioeng., 90(6), 685 (2005)
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Yu H, Stephanopoulos G, Metab. Eng., 10(1), 24 (2008)
Aroskar VJ, Kamat SD, Kamat DV, IIOAB Lett., 2(1) (2012)
