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Received August 12, 2021
Accepted November 19, 2021
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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Enhanced production of biosurfactants through genetic engineering of Pseudozyma sp. SY16
1Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea 2School of Environmental Engineering, University of Seoul, Seoul 02504, Korea 3Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea 4KAIST Institute for BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea 5Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Korea
hkim@kribb.re.kr
Korean Journal of Chemical Engineering, April 2022, 39(4), 997-1003(7), 10.1007/s11814-021-1020-0
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Abstract
Mannosylerythritol lipids (MELs) are natural glycolipids that possess biosurfactant properties and are abundantly produced by Pseudozyma sp. Due to their specific characteristics, such as biodegradability and low toxicity, MELs have attracted significant interest as an alternative to petroleum-based surfactants in medical and cosmetic fields. The present study describes a novel expression system and optimal transformation conditions of Pseudozyma sp. SY16 for producing MELs. The hygromycin resistance gene under the control of Deinococcus radiodurans-derived Kat1 promoter was used as selection marker and the superfolder green fluorescent protein under the control of the yeast glyceraldehyde- 3-phosphate dehydrogenase promoter was used for confirming successful expression. Using this expression system, several transformants overexpressing genes related to MEL production, including emt1, mmf1, and mat1, were generated. Among them, MMF1-2 strain exhibited an MEL yield of 27.9 g/L, which was 31.6% higher than that of the wild-type strain. Altogether, this study demonstrates that engineered yeast strains hold potential for large-scale production of MELs.
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References
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Maier T, Güell M, Serrano L, Febs Lett., 583, 3966 (2009)
Rosano GL, Ceccarelli EA, Front. Microbiol., 5, 172 (2014)
