Articles & Issues

Language: English

Conflict of Interest: In relation to this article, we declare that there is no conflict of interest.

Publication history: Received July 5, 2022
Accepted August 21, 2022

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

All issues

Controlled release of quercetin from HPMC/gellan gum hydrogel for inhibiting melanogenesis in murine melanoma cells

Seulgi Kim^{1 2} Myeongkwan Song^{1 2} Minseon Lee^{1 2} Soonjo Kwon^{1 2†}

¹Department of Biological Engineering, Inha University, Incheon 22212, Korea ²Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Korea

soonjo.kwon@inha.ac.kr

Korean Journal of Chemical Engineering, February 2023, 40(2), 337-343(7), 10.1007/s11814-022-1269-y

Download PDF

Abstract

Although quercetin inhibits melanogenesis, it is cytotoxic at high concentrations. Recent studies have actively investigated carriers for the controlled release of functional drugs at desired concentrations. In this study, porous hydrogel carriers were prepared using hydroxypropyl methylcellulose (HPMC) and gellan gum for the controlled release of quercetin, which acts as a whitening agent. The physical properties and release characteristics of quercetin from HPMC and gellan gum were measured. In a toxicity test using B16F10 melanoma cells, quercetin decreased cell viability at concentrations above 20 μM. Therefore, we used the HPMC (H)/gellan gum (G) hydrogel with H1/G9, H2/G8, H3/G7, H4/G6, and H5/G5 (w/w) ratios at a final solid content of 4% to control quercetin release at a noncytotoxic level. Stiffness of the hydrogel increased, and the pore size became finer with a higher gellan gum content. Quercetin release rate from the HPMC/gellan gum hydrogel decreased with increasing content of gellan gum; for example, the release rate of quercetin from the H1/G9 hydrogel was approximately half of that from the H5/G5 hydrogel. These results suggest that the HPMC/gellan hydrogel may be a useful delivery vehicle to release quercetin, a whitening agent, at non-cytotoxic concentrations.

Keywords

Anti-melanogenesis Quercetin Drug Release HPMC Gellan Gum Hydrogel

References

Brenner M, Hearing VJ, Photochem. Photobiol., 84, 539 (2008)
Choi MH, Shin HJ, Cosmetics, 3, 18 (2016)
Fujii T, Saito M, Biosci. Biotechnol. Biochem., 73, 1989 (2009)
Grasdalen H, Smidsrød O, Carbohydr. Polym., 7, 371 (1987)
Joshi SC, Materials, 4, 1861 (2011)
Kubo I, Kinst-Hori I, J. Agric. Food Chem., 47, 4121 (1999)
Lamson DW, Brignall MS, Alternative Medicine Review: A J. Clinical Therapeutics, 5, 196 (2000)
Lin JW, Chiang HM, Lin YC, Wen KC, J. Food Drug Anal., 16, 75 (2008)
Mitchell K, Ford JL, Armstrong DJ, Elliott PN, Rostron C, Hogan JE, Int. J. Pharm., 66, 233 (1990)
Osmałek T, Froelich A, Tasarek S, Int. J. Pharm., 466, 328 (2014)
Rendon MI, Gaviria JI, Dermatol. Surg., 31, 886 (2005)
Sarkar N, J. Appl. Polym. Sci., 24, 1073 (1979)
Siepmann J, Kranz H, Bodmeier R, Peppas N, Pharm. Res., 16, 1748 (1999)
Siepmann J, Peppas NA, Adv. Drug Deliv. Rev., 64, 163 (2012)
Soppirnath KS, Aminabhavi TM, Eur. J. Pharm. Biopharm., 53, 87 (2002)
Srivastava S, Somasagara RR, Hegde M, Nishana M, Tadi SK, Srivastava M, Choudhary B, Raghavan SC, Sci. Rep., 6, 1 (2016)
Taïeb A, Cario-André M, Briganti S, Picardo M, Melanins and melanosomes: Biosynthesis, structure, physiological and pathological functions, Wiley-Blackwell, Weinheim, Germany (2011).
Tang Y, Sun J, Fan H, Zhang X, Carbohydr. Polym., 88, 46 (2012)
Varghese JS, Chellappa N, Fathima NN, Colloids Surf. B: Biointerfaces, 113, 346 (2014)
Wasmeier C, Hume AN, Bolasco G, Seabra MC, J. Cell Sci., 121, 3995 (2008)
Yang YM, Son YO, Lee SA, Jeon YM, Lee JC, Phytotherapy Res., 25, 1166 (2011)