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Received July 5, 2022
Accepted August 21, 2022
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Effect of direct current electrical stimulation on osteogenic differentiation and calcium influx
1Department of Biological Engineering, Inha University, Incheon 22212, Korea 2Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Korea
soonjo.kwon@inha.ac.kr
Korean Journal of Chemical Engineering, February 2023, 40(2), 344-351(8), 10.1007/s11814-022-1270-5
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Abstract
Electrical stimulation is effective for bone healing. Several studies have shown that electrical stimulation affects cell migration and proliferation. This study examined whether electrical stimulation enhances the osteogenic differentiation of mouse clonal mesenchymal stem cells (cMSCs). Mouse cMSCs were exposed to electrical stimulation at three different voltages (1 V/cm, 0.5 V/cm, and 0.05 V/cm) for one hour per day. The applied electrical stimulation did not affect the cell viability. The alkaline phosphatase (ALP) activity was increased significantly at day 14 after electrical stimulation with 0.5 V/cm for one hour per day. The genes related to osteogenic differentiation were examined at days three, seven, and 14 after exposure to the three electrical stimuli. Osteocalcin (OC) expression was increased dramatically under all three-voltage conditions. These increases were closely related to the electrically stimulated increases in the intracellular calcium concentrations, assessed by Fluo-4 AM on day 14 after exposure to 0.05 V/cm for one hour per day. Furthermore, the expression of calcineurin/NFAT pathway-related genes was upregulated significantly after electrical stimulation. Thus, electrical stimulation enhances the osteogenic differentiation of mouse cMSCs by increasing the intracellular calcium concentration.
References
Miranda M, Moon M, Surg. Treat. Orthop. Trauma, 1, 77 (2007)
Amini AR, Laurencin CT, Nukavarapu SP, Crit. Rev. Biomed. Eng., 40, 363 (2012)
Hierholzer C, Sama D, Toro JB, Peterson M, Helfet DL, J. Bone Jt. Surg.- Ser. A, 88, 1442 (2006)
Ong CT, Choon DSK, Cabrera NP, Maffulli N, Injury, 33, 829 (2002)
Ristiniemi J, Flinkkilä T, Hyvönen P, Lakovaara M, Pakarinen H, Jalovaara P, J. Bone Jt. Surg. - Ser. B, 89, 265 (2007)
Zhang C, Li J, Zhang L, Zhou Y, Hou W, Quan H, Li X, Chen Y, Yu H, Arch. Oral Biol., 57, 1395 (2012)
Mauney JR, Sjostorm S, Blumberg J, Horan R, O’Leary JP, Vunjak-Novakovic G, Volloch V, Kaplan DL, Calcif. Tissue Int., 74, 458 (2004)
Bez M, Sheyn D, Tawackoli W, Avalos P, Shapiro G, Giaconi JC, Da X, David SB, Gavrity J, Awad HA, Bae HW, Sci. Transl. Med., 9, 1 (2017)
Pietronave S, Zamperone A, Oltolina F, Colangelo D, Follenzi A, Novelli E, Diena M, Pavesi A, Consolo F, Fiore GB, Stem Cells Dev., 23, 888 (2014)
Fomby P, Cherlin AJ, Hadjizadeh A, Doillon CJ, Sueblinvong V, Weiss DJ, Bates JHT, Gilbert T, Liles WC, Lutzko C, Ann. Am. Thorac. Soc., 12, 181 (2010)
Kubota K, Yoshimura N, Yokota M, Fitzsimmons RJ, Wikesjö UME, J. Periodontol., 66, 2 (1995)
Balint R, Cassidy NJ, Cartmell SH, Tissue Eng. - Part B Rev., 19, 48 (2013)
Kim IS, Song JK, Song YM, Cho TH, Lee TH, Lim SS, Kim SJ, Hwang SJ, Tissue Eng. Part A, 15, 2411 (2009)
Song SU, Kim CS, Yoon SP, Kim SK, Lee MH, Kang JS, Choi GS, Moon SH, Choi MS, Cho YK, Son BK, Stem Cells Dev., 17, 451 (2008)
Yi TG, Kim SN, Lee HJ, Kim J, Cho YK, Shin DH, Tak SJ, Moon SH, Kang JE, Ji IM, Lim HJ, Lee DS, Jeon MS, Tissue Eng. - Part C Methods, 21, 1251 (2015)
Mobini S, Leppik L, Barker JH, Biotechniques, 60, 95 (2016)
Mobini S, Leppik L, Parameswaran VT, Barker JH, PeerJ, 2017, e2821 (2017)
Srirussamee K, Mobini S, Cassidy NJ, Cartmell SH, Biotechnol. Bioeng., 116, 3421 (2019)
Ju C, Park E, Kim T, Kim T, Kang M, Lee KS, Park SM, PLoS One, 15, 1 (2020)
Tandon N, Cannizzaro C, Chao PHG, Maidhof R, Marsano A, Au HTH, Radisic M, Vunjak-Novakovic G, Nat. Protoc., 4, 155 (2009)
Beugels J, Molin DGM, Ophelders DRMG, Rutten T, Kessels L, Kloosterboer N, de Grzymala AAP, Kramer BWW, Sci. Rep., 9, 1 (2019)
Leppik L, Oliveira KMC, Bhavsar MB, Barker JH, Eur. J. Trauma Emerg. Surg., 46, 231 (2020)
Wang ET, Zhao M, Chinese J. Traumatol. - English Ed., 13, 55 (2010)
Cortese B, Palamà IE, D’Amone S, Gigli G, Integr. Biol., 6, 817 (2014)
Rajabi AH, Jaffe M, Arinzeh TL, Acta Biomater., 24, 12 (2015)
McCullen SD, McQuilling JP, Grossfeld RM, Lubischer JL, Clarke LI, Loboa EG, Tissue Eng. - Part C Methods, 16, 1377 (2010)
An S, Gao Y, Ling J, Wei X, Xiao Y, J. Mater. Sci. -Mater. Med., 23, 789 (2012)
Bonnet N, Calcif. Tissue Int., 100, 174 (2017)
Tsao YT, Huang YJ, Wu HH, Liu YA, Liu YS, Lee OK, Int. J. Mol. Sci., 18, 159 (2017)
Winslow MM, Pan M, Starbuck M, Gallo EM, Deng L, Karsenty G, Crabtree GR, Dev. Cell, 10, 771 (2006)
Leppik L, Zhihua H, Mobini S, Parameswaran VT, Eischen-Loges M, Slavici A, Helbing J, Pindur L, Oliveira KMC, Sci. Rep., 8, 1 (2018)
Srirussamee K, Xue R, Mobini S, Cassidy NJ, Cartmell SH, J. Tissue Eng., 12, 20417314 (2021)
Amini AR, Laurencin CT, Nukavarapu SP, Crit. Rev. Biomed. Eng., 40, 363 (2012)
Hierholzer C, Sama D, Toro JB, Peterson M, Helfet DL, J. Bone Jt. Surg.- Ser. A, 88, 1442 (2006)
Ong CT, Choon DSK, Cabrera NP, Maffulli N, Injury, 33, 829 (2002)
Ristiniemi J, Flinkkilä T, Hyvönen P, Lakovaara M, Pakarinen H, Jalovaara P, J. Bone Jt. Surg. - Ser. B, 89, 265 (2007)
Zhang C, Li J, Zhang L, Zhou Y, Hou W, Quan H, Li X, Chen Y, Yu H, Arch. Oral Biol., 57, 1395 (2012)
Mauney JR, Sjostorm S, Blumberg J, Horan R, O’Leary JP, Vunjak-Novakovic G, Volloch V, Kaplan DL, Calcif. Tissue Int., 74, 458 (2004)
Bez M, Sheyn D, Tawackoli W, Avalos P, Shapiro G, Giaconi JC, Da X, David SB, Gavrity J, Awad HA, Bae HW, Sci. Transl. Med., 9, 1 (2017)
Pietronave S, Zamperone A, Oltolina F, Colangelo D, Follenzi A, Novelli E, Diena M, Pavesi A, Consolo F, Fiore GB, Stem Cells Dev., 23, 888 (2014)
Fomby P, Cherlin AJ, Hadjizadeh A, Doillon CJ, Sueblinvong V, Weiss DJ, Bates JHT, Gilbert T, Liles WC, Lutzko C, Ann. Am. Thorac. Soc., 12, 181 (2010)
Kubota K, Yoshimura N, Yokota M, Fitzsimmons RJ, Wikesjö UME, J. Periodontol., 66, 2 (1995)
Balint R, Cassidy NJ, Cartmell SH, Tissue Eng. - Part B Rev., 19, 48 (2013)
Kim IS, Song JK, Song YM, Cho TH, Lee TH, Lim SS, Kim SJ, Hwang SJ, Tissue Eng. Part A, 15, 2411 (2009)
Song SU, Kim CS, Yoon SP, Kim SK, Lee MH, Kang JS, Choi GS, Moon SH, Choi MS, Cho YK, Son BK, Stem Cells Dev., 17, 451 (2008)
Yi TG, Kim SN, Lee HJ, Kim J, Cho YK, Shin DH, Tak SJ, Moon SH, Kang JE, Ji IM, Lim HJ, Lee DS, Jeon MS, Tissue Eng. - Part C Methods, 21, 1251 (2015)
Mobini S, Leppik L, Barker JH, Biotechniques, 60, 95 (2016)
Mobini S, Leppik L, Parameswaran VT, Barker JH, PeerJ, 2017, e2821 (2017)
Srirussamee K, Mobini S, Cassidy NJ, Cartmell SH, Biotechnol. Bioeng., 116, 3421 (2019)
Ju C, Park E, Kim T, Kim T, Kang M, Lee KS, Park SM, PLoS One, 15, 1 (2020)
Tandon N, Cannizzaro C, Chao PHG, Maidhof R, Marsano A, Au HTH, Radisic M, Vunjak-Novakovic G, Nat. Protoc., 4, 155 (2009)
Beugels J, Molin DGM, Ophelders DRMG, Rutten T, Kessels L, Kloosterboer N, de Grzymala AAP, Kramer BWW, Sci. Rep., 9, 1 (2019)
Leppik L, Oliveira KMC, Bhavsar MB, Barker JH, Eur. J. Trauma Emerg. Surg., 46, 231 (2020)
Wang ET, Zhao M, Chinese J. Traumatol. - English Ed., 13, 55 (2010)
Cortese B, Palamà IE, D’Amone S, Gigli G, Integr. Biol., 6, 817 (2014)
Rajabi AH, Jaffe M, Arinzeh TL, Acta Biomater., 24, 12 (2015)
McCullen SD, McQuilling JP, Grossfeld RM, Lubischer JL, Clarke LI, Loboa EG, Tissue Eng. - Part C Methods, 16, 1377 (2010)
An S, Gao Y, Ling J, Wei X, Xiao Y, J. Mater. Sci. -Mater. Med., 23, 789 (2012)
Bonnet N, Calcif. Tissue Int., 100, 174 (2017)
Tsao YT, Huang YJ, Wu HH, Liu YA, Liu YS, Lee OK, Int. J. Mol. Sci., 18, 159 (2017)
Winslow MM, Pan M, Starbuck M, Gallo EM, Deng L, Karsenty G, Crabtree GR, Dev. Cell, 10, 771 (2006)
Leppik L, Zhihua H, Mobini S, Parameswaran VT, Eischen-Loges M, Slavici A, Helbing J, Pindur L, Oliveira KMC, Sci. Rep., 8, 1 (2018)
Srirussamee K, Xue R, Mobini S, Cassidy NJ, Cartmell SH, J. Tissue Eng., 12, 20417314 (2021)
