Articles & Issues
- Language
- English
- Conflict of Interest
- In relation to this article, we declare that there is no conflict of interest.
- Publication history
-
Received July 12, 2022
Accepted September 30, 2022
-
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Copyright © KIChE. All rights reserved.
All issues
Development of a liquid-based cytology method for detecting cervical cancer cells using functional gold nanorods
Eunseo Jeong1
Jongjun Park1
Hayoung Kim1
Sungjun Lee1 2
Yonghyun Choi1 2
Masayoshi Tanaka3
Jonghoon Choi1 2†
1School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea 2Feynman Institute of Technology, Nanomedicine Corporation, Seoul 06974, Korea 3Department of Chemical Science and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8503, Japan
nanomed@cau.ac.kr
Korean Journal of Chemical Engineering, February 2023, 40(2), 369-378(10), 10.1007/s11814-022-1307-9
Download PDF
Abstract
A cytopathology test for cervical cancer diagnosis has been introduced to complement the histopathology test. This study developed a new cervical cancer diagnostic method that complements the existing liquid cytology to reduce the diagnostician’s skill dependence and to improve diagnostic accuracy. With the ease of introduction of surface functional groups and their high biocompatibility and stability, gold nanomaterials have been used to diagnose numerous biomolecules. CA125 and p16 markers were chosen as protein markers uniquely present in cervical cancer. Bioconjugation technology was used to form a stable bond between the target antibody and gold nanorods. Antibodygold nanorods were administered to cervical cancer cells as a factor for optical microscopic analysis. The degree of cancer cell labeling was determined through additional fluorescence microscopy analysis. Additionally, a liquid-based cytology test mimicking the clinical environment was prepared, and the false-positive rate, false-negative rate, and accuracy were determined when the study strategy was implemented. The strategy validated in this study (antibody-gold nanorodbased cervical cancer diagnosis) suggests a method that can reduce the dependence on experts for the cancer cytology and can increase test accuracy.
References
Waggoner SE, Lancet, 361, 9376 (2003)
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A, Cancer J. Clinicians, 68, 6 (2018)
Cohen PA, Jhingran A, Oaknin A, Denny L, Lancet, 393, 10167 (2019)
Duenas-Gonzalez A, Lizano M, Candelaria M, Cetina L, Arce C, Cervera E, Mol. Cancer, 4, 1 (2005)
Kessler TA, Semin Oncol Nurs., 33, 2 (2017)
Dasari S, Wudayagiri R, Valluru L, Clin. Chim. Acta, 445, 7 (2015)
Madrid-Marina V, Torres-Poveda K, Lopez-Toledo G, Garcia-Carranca A, Arch. Med. Res., 40, 6 (2009)
Harper DM, Franco EL, Wheeler C, Ferris DG, Jenkins D, Schuind A, Zahaf T, Innis B, Naud P, De Carvalho NS, Lancet, 364, 9447 (2004)
Koutsky LA, Ault KA, Wheeler CM, Brown DR, Barr E, Alvarez FB, Chiacchierini LM, Jansen KU, N. Engl. J. Med., 347, 1645 (2002)
Wentzensen N, Sherman ME, Schiffman M, Wang SS, Gynecol Oncol., 112, 293 (2009)
Shingleton HM, Patrick RL, Johnston WW, Smith RA, CA Cancer J. Clin., 45, 5 (1995)
Papanicolaou GN, Traut HF, Arch. Pathol. Lab. Med., 121, 3 (1997)
Khan MS, Raja FY, Ishfaq G, Tahir F, Subhan F, Kazi BM, Karamat KA, Pak. J. Med. Res., 44, 111 (2005)
Cronje HS, Int. J. Gynaecol Obstet., 84, 101 (2004)
Gibb RK, Martens MG, Rev. Obstet. Gynecol., 4, S2 (2011)
Gabriel C, Achten R, Drijkoningen M, Acta Cytol., 48, 6 (2004)
Singh U, Qureshi S, Negi N, Singh N, Goel M, Srivastava K, Ind. J. Med. Res., 147, 3 (2018)
Jin J, Yue CY, J. Lab. Med., 44, 3 (2020)
de Bekker-Grob EW, de Kok IM, Bulten J, van Rosmalen J, Vedder JE, Arbyn M, Klinkhamer PJ, Siebers AG, van Ballegooijen M, Cancer Causes Control, 23, 8 (2012)
Dykman L, Khlebtsov N, Chem. Soc. Rev., 41, 6 (2012)
Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA, Chem. Soc. Rev., 41, 7 (2012)
Boisselier E, Astruc D, Chem. Soc. Rev., 38, 6 (2009)
Newhouse RJ, Zhang JZ, Reviews in Plasmonics 2010, Springer Nature, Switzerland (2012).
Mout R, Moyano DF, Rana S, Rotello VM, Chem. Soc. Rev., 41, 7 (2012)
Dykman LA, Khlebtsov NG, Chem. Rev., 114, 2 (2014)
Burgi T, Nanoscale, 7, 38 (2015)
Jazayeri MH, Amani H, Pourfatollah AA, Pazoki-Toroudi H, Sedighimoghaddam B, Sensing Bio-sensing Research, 9, 17 (2016)
Goldberg GL, Sklar A, O'Hanlan KA, Levine PA, Runowicz CD, Gynecol Oncol., 40, 3 (1991)
Shen H, Guo M, Wang L, Cui X, Genes Genomics, 42, 2 (2020)
Bender DP, Sorosky JI, Buller RE, Sood AK, Am. J. Obstet. Gynecol., 189, 1 (2003)
Porika M, Vemunoori AK, Tippani R, Mohammad A, Bollam SR, Abbagani S, Asian Pac. J. Cancer Prev., 11, 6 (2010)
Zhou LL, Shen Y, Gong JM, Sun P, Sheng JH, Oncotarget, 8, 41 (2017)
Crawford A, Haber L, Kelly MP, Vazzana K, Canova L, Ram P, Pawashe A, Finney J, Jalal S, Chiu D, Colleton CA, Garnova E, Makonnen S, Sci. Transl. Med., 11, 497 (2019)
Roelens J, Reuschenbach M, von Knebel Doeberitz M, Wentzensen N, Bergeron C, Arbyn M, Cancer Cytopathol., 120, 5 (2012)
Romagosa C, Simonetti S, Lopez-Vicente L, Mazo A, Lleonart ME, Castellvi J, y Cajal SR, Oncogene, 30, 18 (2011)
Munger K, Werness BA, Dyson N, Phelps WC, Harlow E, Howley PM, Embo J., 8, 13 (1989)
Reuschenbach M, Waterboer T, Wallin KL, Einenkel J, Dillner J, Hamsikova E, Eschenbach D, Zimmer H, Heilig B, Int. J. Cancer, 123, 11 (2008)
Haller F, Agaimy A, Cameron S, Beyer M, Gunawan B, Happel N, Langer C, Ramadori G, von Heydebreck A, Fuzesi L, Histopathology, 56, 3 (2010)
Bastide K, Guilly MN, Bernaudin JF, Joubert C, Lectard B, Levalois C, Malfoy B, Chevillard S, Lung Cancer, 63, 3 (2009)
Schwabe M, Lubbert M, Curr. Pharm. Biotechnol., 8, 6 (2007)
Ye X, Zheng C, Chen J, Gao Y, Murray CB, Nano Lett., 13, 2 (2013)
Monsonego J, Hudgens MG, Zerat L, Zerat JC, Syrjanen K, Halfon P, Ruiz F, Smith JS, Int. J. Cancer, 129, 3 (2011)
Kirschner B, Simonsen K, Junge J, Cytopathology, 17, 4 (2006)
Kaufman HW, Alagia DP, Chen Z, Onisko A, Austin RM, Am. J. Clin. Pathol., 154, 4 (2020)
Tanabodee J, Thepsuwan K, Karalak A, Laoaree O, Krachang A, Manmatt K, Anontwatanawong N, Asian Pac. J. Cancer Prev., 16, 16 (2015)
Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, J. Natl. Cancer Inst., 105, 20 (2013)
Mishra GA, Pimple SA, Shastri SS, Ind. J. Med. Paediatr. Oncol., 32, 3 (2011)
Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A, Cancer J. Clinicians, 68, 6 (2018)
Cohen PA, Jhingran A, Oaknin A, Denny L, Lancet, 393, 10167 (2019)
Duenas-Gonzalez A, Lizano M, Candelaria M, Cetina L, Arce C, Cervera E, Mol. Cancer, 4, 1 (2005)
Kessler TA, Semin Oncol Nurs., 33, 2 (2017)
Dasari S, Wudayagiri R, Valluru L, Clin. Chim. Acta, 445, 7 (2015)
Madrid-Marina V, Torres-Poveda K, Lopez-Toledo G, Garcia-Carranca A, Arch. Med. Res., 40, 6 (2009)
Harper DM, Franco EL, Wheeler C, Ferris DG, Jenkins D, Schuind A, Zahaf T, Innis B, Naud P, De Carvalho NS, Lancet, 364, 9447 (2004)
Koutsky LA, Ault KA, Wheeler CM, Brown DR, Barr E, Alvarez FB, Chiacchierini LM, Jansen KU, N. Engl. J. Med., 347, 1645 (2002)
Wentzensen N, Sherman ME, Schiffman M, Wang SS, Gynecol Oncol., 112, 293 (2009)
Shingleton HM, Patrick RL, Johnston WW, Smith RA, CA Cancer J. Clin., 45, 5 (1995)
Papanicolaou GN, Traut HF, Arch. Pathol. Lab. Med., 121, 3 (1997)
Khan MS, Raja FY, Ishfaq G, Tahir F, Subhan F, Kazi BM, Karamat KA, Pak. J. Med. Res., 44, 111 (2005)
Cronje HS, Int. J. Gynaecol Obstet., 84, 101 (2004)
Gibb RK, Martens MG, Rev. Obstet. Gynecol., 4, S2 (2011)
Gabriel C, Achten R, Drijkoningen M, Acta Cytol., 48, 6 (2004)
Singh U, Qureshi S, Negi N, Singh N, Goel M, Srivastava K, Ind. J. Med. Res., 147, 3 (2018)
Jin J, Yue CY, J. Lab. Med., 44, 3 (2020)
de Bekker-Grob EW, de Kok IM, Bulten J, van Rosmalen J, Vedder JE, Arbyn M, Klinkhamer PJ, Siebers AG, van Ballegooijen M, Cancer Causes Control, 23, 8 (2012)
Dykman L, Khlebtsov N, Chem. Soc. Rev., 41, 6 (2012)
Dreaden EC, Alkilany AM, Huang X, Murphy CJ, El-Sayed MA, Chem. Soc. Rev., 41, 7 (2012)
Boisselier E, Astruc D, Chem. Soc. Rev., 38, 6 (2009)
Newhouse RJ, Zhang JZ, Reviews in Plasmonics 2010, Springer Nature, Switzerland (2012).
Mout R, Moyano DF, Rana S, Rotello VM, Chem. Soc. Rev., 41, 7 (2012)
Dykman LA, Khlebtsov NG, Chem. Rev., 114, 2 (2014)
Burgi T, Nanoscale, 7, 38 (2015)
Jazayeri MH, Amani H, Pourfatollah AA, Pazoki-Toroudi H, Sedighimoghaddam B, Sensing Bio-sensing Research, 9, 17 (2016)
Goldberg GL, Sklar A, O'Hanlan KA, Levine PA, Runowicz CD, Gynecol Oncol., 40, 3 (1991)
Shen H, Guo M, Wang L, Cui X, Genes Genomics, 42, 2 (2020)
Bender DP, Sorosky JI, Buller RE, Sood AK, Am. J. Obstet. Gynecol., 189, 1 (2003)
Porika M, Vemunoori AK, Tippani R, Mohammad A, Bollam SR, Abbagani S, Asian Pac. J. Cancer Prev., 11, 6 (2010)
Zhou LL, Shen Y, Gong JM, Sun P, Sheng JH, Oncotarget, 8, 41 (2017)
Crawford A, Haber L, Kelly MP, Vazzana K, Canova L, Ram P, Pawashe A, Finney J, Jalal S, Chiu D, Colleton CA, Garnova E, Makonnen S, Sci. Transl. Med., 11, 497 (2019)
Roelens J, Reuschenbach M, von Knebel Doeberitz M, Wentzensen N, Bergeron C, Arbyn M, Cancer Cytopathol., 120, 5 (2012)
Romagosa C, Simonetti S, Lopez-Vicente L, Mazo A, Lleonart ME, Castellvi J, y Cajal SR, Oncogene, 30, 18 (2011)
Munger K, Werness BA, Dyson N, Phelps WC, Harlow E, Howley PM, Embo J., 8, 13 (1989)
Reuschenbach M, Waterboer T, Wallin KL, Einenkel J, Dillner J, Hamsikova E, Eschenbach D, Zimmer H, Heilig B, Int. J. Cancer, 123, 11 (2008)
Haller F, Agaimy A, Cameron S, Beyer M, Gunawan B, Happel N, Langer C, Ramadori G, von Heydebreck A, Fuzesi L, Histopathology, 56, 3 (2010)
Bastide K, Guilly MN, Bernaudin JF, Joubert C, Lectard B, Levalois C, Malfoy B, Chevillard S, Lung Cancer, 63, 3 (2009)
Schwabe M, Lubbert M, Curr. Pharm. Biotechnol., 8, 6 (2007)
Ye X, Zheng C, Chen J, Gao Y, Murray CB, Nano Lett., 13, 2 (2013)
Monsonego J, Hudgens MG, Zerat L, Zerat JC, Syrjanen K, Halfon P, Ruiz F, Smith JS, Int. J. Cancer, 129, 3 (2011)
Kirschner B, Simonsen K, Junge J, Cytopathology, 17, 4 (2006)
Kaufman HW, Alagia DP, Chen Z, Onisko A, Austin RM, Am. J. Clin. Pathol., 154, 4 (2020)
Tanabodee J, Thepsuwan K, Karalak A, Laoaree O, Krachang A, Manmatt K, Anontwatanawong N, Asian Pac. J. Cancer Prev., 16, 16 (2015)
Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, J. Natl. Cancer Inst., 105, 20 (2013)
Mishra GA, Pimple SA, Shastri SS, Ind. J. Med. Paediatr. Oncol., 32, 3 (2011)
