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Received August 1, 2022
Accepted November 16, 2022
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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0) which permits
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A high-sensitivity cfDNA capture enables to detect the BRAF V600E mutation in papillary thyroid carcinoma
Tae Hee Lee1 2
Hong Jae Jeon3
Jung Hyun Choi4
Young Jun Kim5
Pil-Neo Hwangbo6
Hyun Sung Park4
Chae Yeon Son4
Hei-Gwon Choi7 8
Ha Neul Kim7 8
Jae Won Chang9
Jiyoon Bu4 10†
Hyuk Soo Eun11 12†
1Department of Biomedical Laboratory Science, Daegu Health College, Chang-ui Building, 15 Yeongsong-ro, Buk-gu, Daegu 41453, Korea 2Industry Academic Cooperation Foundation,, Daegu Health College, 15 Yeongsong-ro, Buk-gu, Daegu 41453, Korea 3Department of Internal Medicine, Chungnam National University Sejong Hospital (CNUSH), 20, Bodeum 7-ro, Sejong 30099, Korea 4Department of Biological Sciences and Bioengineering, Inha University, 100 Inha-ro, Michuhol-gu, Incheon 22212, Korea 5School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 06974, Korea 6Industry Academic Cooperation Foundation, Daegu Health College, 15 Yeongsong-ro, Buk-gu, Daegu 41453, Korea 7Brain Korea 21 FOUR Project for Medical Science, Chungnam National University, 266, Munwha-ro, jung-gu, Daejeon 35015, Korea 8Department of Medical Science, Chungnam National University, 266, Munwha-ro, jung-gu, Daejeon 35015, Korea 9Department of Otolaryngology-Head and Neck Surgery, Research Institute for Medical Science,, Chungnam National University, 266, School of Medicine, Daejeon 35015, Korea 10Industry-Academia Interactive R&E Center for Bioprocess Innovation, Inha University, Incheon 22212, Korea 11Department of Internal Medicine, Chungnam National University Hospital, 282, Daejeon 35015, Korea 12Department of Internal Medicine, College of Medicine, Chungnam National University, 266, Munwha-ro, jung-gu, Daejeon 35015, Korea
jbu@inha.ac.kr
Korean Journal of Chemical Engineering, February 2023, 40(2), 429-435(7), 10.1007/s11814-022-1348-0
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Abstract
Although many efforts have been made to investigate a promising biomarker for the diagnosis of papillary thyroid cancer (PTC), the currently available biomarkers lack the sensitivity to accurately characterize PTC. Here, we established a high-sensitivityt cell-free DNA (cfDNA) detection system using polydopamine-silica (PDA/SiO2) hybrids to enable clinically reliable analysis of PTC. The PDA/SiO2-coated beads improved the detection of DNA by 1.76-fold (p=0.031) compared to the conventional silica-based cfDNA capture system. The PDA-SiO2-coated beads were then applied for the detection of cfDNA from serum samples obtained from 37 PTC patients, and the BRAFV600E mutation status in captured DNA was analyzed using both quantitative polymerase chain reaction (qPCR) and digital droplet PCR (ddPCR). The BRAFV600E mutation status analyzed using both assays demonstrated a strong correlation with the multifocality of PTC, exhibiting area under receiver operating characteristic curve (AUC-ROC) of >0.964 (p<0.001). In contrast, none of the serum antigens or antibodies related to PTC or thyroid functions exhibited clinically significant prediction for detecting PTC patients with multifocal tumors. These findings suggest that cfDNA capture using PDA/ SiO2-coated beads is a promising approach for analyzing the BRAF mutation, which can serve as an excellent diagnostic biomarker for PTC.
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Xing M, Westra WH, Tufano RP, Cohen Y, Rosenbaum E, Rhoden KJ, Carson KA, Vasko V, Larin A, Tallini G, Tolaney S, J. Clin. Endocrinol. Metab., 90(12), 6373 (2005)
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Orell SR, Cytopathology, 14(4), 173 (2003)
Abi-Raad R, Prasad M, Baldassari R, Schofield K, Callender GG, Chhieng D, Adeniran AJ, Endocr. Pathol., 29(3), 269 (2018)
Yu XM, Patel PN, Chen H, Sippel RS, Am. J. Surg., 203(3), 331 (2012)
Campbell MJ, Seib CD, Candell L, Gosnell JE, Duh QY, Clark OH, Shen WT, World J. Surg., 39(3), 695 (2015)
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Lee T, Rawding PA, Bu J, Hyun S, Rou W, Jeon H, Kim S, Lee B, Kubiatowicz LJ, Kim D, Hong S, Eun H, Cancers, 14(9), 2061 (2022)
Bu J, Lee TH, Jeong WJ, Poellmann MJ, Mudd K, Eun HS, Liu EW, Hong S, Hyun SH, PloS One, 15(12), e0242145 (2020)
Kimbrell HA, Sholl AB, Ratnayaka S, Japa S, Lacey M, Carpio G, Bhatia P, Kandil E, BioMed. Res. Int., 2015, 486391 (2015)
Li C, Lee KC, Schneider EB, Zeiger MA, J. Clin. Endocrinol. Metab., 97(12), 4559 (2012)
Jeong D, Jeong Y, Park JH, Han SW, Kim SY, Kim YJ, Kim SJ, Hwangbo Y, Park S, Cho HD, Oh MH, Yang SH, Kim CJ, Ann. Surg. Oncol., 20(3), 759 (2013)
Bu J, Lee TH, Poellmann MJ, Rawding PA, Jeong WJ, Hong RS, Hyun SH, Eun HS, Hong S, Clin. Transl. Med., 11(8), e499 (2021)
Prpić M, Franceschi M, Romić M, Jukić T, Kusić Z, Acta Clinica Croat., 57(3), 518 (2018)
Indrasena BSH, World J. Biol. Chem., 8(1), 81 (2017)
Zhao CK, Zheng JY, Sun LP, Xu RY, Wei Q, Xu HX, Cancer Med., 8(12), 5577 (2019)
