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- In relation to this article, we declare that there is no conflict of interest.
- Publication history
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Received June 29, 2022
Revised November 6, 2022
Accepted November 16, 2022
- Acknowledgements
- This work was supported by the National Natural Science Foundation of China (No. 52170034).
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Variation in the quantity and composition of phosphorus accumulating organisms in activated sludge driven by nitrate-nitrogen
Key Laboratory of Songliao Aquatic Environment, Ministry of Education, Jilin Jianzhu University, Changchun City, Jilin Province, P. R. Chin
wangxiaoling1977@126.com
Korean Journal of Chemical Engineering, July 2023, 40(7), 1661-1671(11), 10.1007/s11814-022-1349-z
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Abstract
Anaerobic/anoxia sequencing batch reactor (A/ASBR) system was used to analyze the quantity and composition of each branch of phosphorus accumulating organisms (PAOs) in activated sludge under different nitratenitrogen (NO3
-N) concentrations by using real-time quantitative polymerase chain reaction (PCR) technology. The
study determined whether NO3
-N and its concentration change were the main driving factors for the variation of the
quantity and composition of each branch of PAOs. The results show that with the increase of NO3
-N concentration
from 10 mg/L to 40 mg/L, the number of bacterial 16S rRNA genes in the A/ASBR reactor changed slightly at 6.81×
1011-7.53×1011 copies/g dry sludge. The number of PAO genes (Acc 16S rRNA) increased from 1.98×1011 to 3.53×1011
copies/g dry sludge, and the total number of ppk1 genes increased from 1.25×1011 to 3.59×1011 copies/g dry sludge.
Additionally, the number of polyphosphate kinase (ppk) genes in Accumulibacter branch IA, IIC and IID was high,
and the changes were positively related to the concentration of NO3
-N, while the number of branches in IIA, IIB and
IIF was very low. The dosing concentration of NO3
-N was the main driving factor for the change of PAOs and their
branch number and composition in the A/ASBR reactor.
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University of Technology, Beijing (2013).
43. Y. P. Mao, D. W. Graham, H. Tamaki and T. Zhang, Sci. Rep., 46,11857 (2015).
44. Z. R. Hu, M. C. Wentzel and G. A. Ekama, Water Res., 36, 4927(2002).
45. W. Zeng, X. L. Bai, Y. Guo, N. Li and Y. Z. Peng, Enzyme Microb.Tech., 105, 1 (2017).
46. W. Zeng, B. X. Li, X. D. Wang, X. L. Bai and Y. Z. Peng, Chemosphere, 144, 1018 (2016).
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48. C. T. Skennerton, J. J. Barr, F. R. Slater, P. L. Bond and G. W. Tyson,Environ. Microbiol., 17, 1574 (2015).
49. J. J. Flowers, S. He, S. Yilmaz, D. R. Noguera and K. D. McMahon,Env. Microbiol. Rep., 1, 583 (2009).
50. G. Carvalho, P. C. Lemos, A. Oehmen and M. A. M. Reis, Water Res., 41, 4383 (2007)
