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Received November 15, 2022
Revised January 18, 2023
Accepted January 31, 2023
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Molecular encapsulation of nortriptyline in the -cyclodextrin cavity: In-vitro cytotoxic potential against MCF-7 cell line
Rajaram Rajamohan1†
Muthusamy Viswalingam2†
Yong Rok Lee1†
Samikannu Prabu3
Krishnamoorthy Sivakumar4
1School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea 2Department of Chemistry, Periyar Maniammai Institute of Science and Technology, Thanjavur - 613 403, India 3Department of Chemistry, P.S.V College of Engineering and Technology, Krishnagiri - 635 108, India 4Department of Chemistry, Sri Chandrasekharendra Saraswathi Viswa Mahavidyalaya (Deemed University), Kanchipuram - 631 561, India
Korean Journal of Chemical Engineering, July 2023, 40(7), 1715-1724(10), 10.1007/s11814-023-1399-x
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Abstract
In the liquid state, UV-visible and fluorescence spectroscopy was used to examine the inclusion complexes
of nortriptyline (NP) and -cyclodextrin (-CD). The degree of inclusion complexation causes NP’s absorbance and
fluorescence intensity to be significantly increased during interaction with -CD. The binding constant was determined by UV-VIS and fluorescence spectroscopy, and the results indicated a 1 : 1 stoichiometry for the inclusion complex at 303 K. Complexation is a spontaneous and exothermic process, as determined by Gibbs’s free energy change. To
produce solid inclusion complexes (ICs), mixing and co-precipitation were used, which were then characterized using
Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), X-ray powder diffraction
(XRD), and thermogravimetric analysis/differential scanning calorimetry (TGA/DSC). According to molecular docking studies, the aromatic ring of the NP does not penetrate the secondary hydroxyl rim of the -CD cavity, but the aliphatic part of the NP trapped in the cavity is more thermodynamically advantageous. NP and NP : -CD-ICs were
screened for in vitro cytotoxicity on Michigan Cancer Foundation-7 (MCF-7) cell line using the 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, and the results showed that the cytotoxicity was not affected
by creating an ICs
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50. M. L. Connolly, Science, 221, 709 (1983)
